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题名: Study of enantioselective interactions between chiral drugs and serum albumin by capillary electrophoresis
作者: Zhu, XF;  Ding, YS;  Lin, BC;  Jakob, A;  Koppenhoefer, B
关键词: capillary electrophoresis ;  chiral separation ;  protein ;  interaction ;  stereoselectivity
刊名: ELECTROPHORESIS
发表日期: 1999-07-01
卷: 20, 期:9, 页:1869-1877
收录类别: ISTP ;  SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The separation of the enantiomers of three basic drugs, i.e., ofloxacin, propranolol and verapamil, was achieved by affinity capillary electrophoresis (ACE), with human serum albumin (HSA) and bovine serum albumin (BSA) as chiral selectors in phosphate buffer at pH 7.4. Ofloxacin was only separated in the presence of BSA, and verapamil only with HSA, while propranolol was separated with either HSA or BSA. The effects of protein concentration and column wall adsorption on the degree of separation were investigated. Two displacers, ketoprofen and warfarin, respectively, when added to the protein containing buffer, both showed significant effects on the separation behavior. From these data it was argued that verapamil may bind to HSA at both locations known, the warfarin binding site (I) and the ketoprofen binding site (II). While with BSA, binding of ofloxacin may also occur at site I, the preferential binding site for propanolol remains controversial. A drug-drug interaction between propranolol and ketoprofen due to opposite charges was concluded from the increase in migration time in BSA solution. The unbound concentration of verapamil enantiomers in solution in the presence of HSA, as estimated from CD-modified capillary zone electrophoresis, was triggered not only by the HSA concentration but also by the coadditive concentration.
关键词[WOS]: PARTIAL-FILLING TECHNIQUE ;  AFFINITY ELECTROKINETIC CHROMATOGRAPHY ;  HUMAN ALPHA(1)-ACID GLYCOPROTEIN ;  ZONE-ELECTROPHORESIS ;  PROTEIN-BINDING ;  STEREOSELECTIVE BINDING ;  KETOPROFEN ENANTIOMERS ;  FRONTAL ANALYSIS ;  SOLVATING AGENT ;  BASIC DRUG
语种: 英语
WOS记录号: WOS:000081688800011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138652
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Tubingen, Inst Organ Chem, D-72074 Tubingen, Germany

Recommended Citation:
Zhu, XF,Ding, YS,Lin, BC,et al. Study of enantioselective interactions between chiral drugs and serum albumin by capillary electrophoresis[J]. ELECTROPHORESIS,1999,20(9):1869-1877.
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