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Study of enantioselective interactions between chiral drugs and serum albumin by capillary electrophoresis
Zhu, XF; Ding, YS; Lin, BC; Jakob, A; Koppenhoefer, B
关键词Capillary Electrophoresis Chiral Separation Protein Interaction Stereoselectivity
刊名ELECTROPHORESIS
1999-07-01
20期:9页:1869-1877
收录类别ISTP ; SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]PARTIAL-FILLING TECHNIQUE ; AFFINITY ELECTROKINETIC CHROMATOGRAPHY ; HUMAN ALPHA(1)-ACID GLYCOPROTEIN ; ZONE-ELECTROPHORESIS ; PROTEIN-BINDING ; STEREOSELECTIVE BINDING ; KETOPROFEN ENANTIOMERS ; FRONTAL ANALYSIS ; SOLVATING AGENT ; BASIC DRUG
英文摘要The separation of the enantiomers of three basic drugs, i.e., ofloxacin, propranolol and verapamil, was achieved by affinity capillary electrophoresis (ACE), with human serum albumin (HSA) and bovine serum albumin (BSA) as chiral selectors in phosphate buffer at pH 7.4. Ofloxacin was only separated in the presence of BSA, and verapamil only with HSA, while propranolol was separated with either HSA or BSA. The effects of protein concentration and column wall adsorption on the degree of separation were investigated. Two displacers, ketoprofen and warfarin, respectively, when added to the protein containing buffer, both showed significant effects on the separation behavior. From these data it was argued that verapamil may bind to HSA at both locations known, the warfarin binding site (I) and the ketoprofen binding site (II). While with BSA, binding of ofloxacin may also occur at site I, the preferential binding site for propanolol remains controversial. A drug-drug interaction between propranolol and ketoprofen due to opposite charges was concluded from the increase in migration time in BSA solution. The unbound concentration of verapamil enantiomers in solution in the presence of HSA, as estimated from CD-modified capillary zone electrophoresis, was triggered not only by the HSA concentration but also by the coadditive concentration.
语种英语
WOS记录号WOS:000081688800011
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被引频次:45[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/138652
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Tubingen, Inst Organ Chem, D-72074 Tubingen, Germany
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GB/T 7714
Zhu, XF,Ding, YS,Lin, BC,et al. Study of enantioselective interactions between chiral drugs and serum albumin by capillary electrophoresis[J]. ELECTROPHORESIS,1999,20(9):1869-1877.
APA Zhu, XF,Ding, YS,Lin, BC,Jakob, A,&Koppenhoefer, B.(1999).Study of enantioselective interactions between chiral drugs and serum albumin by capillary electrophoresis.ELECTROPHORESIS,20(9),1869-1877.
MLA Zhu, XF,et al."Study of enantioselective interactions between chiral drugs and serum albumin by capillary electrophoresis".ELECTROPHORESIS 20.9(1999):1869-1877.
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