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Study of enantioselective interactions between chiral drugs and serum albumin by capillary electrophoresis
Zhu, XF; Ding, YS; Lin, BC; Jakob, A; Koppenhoefer, B
KeywordCapillary Electrophoresis Chiral Separation Protein Interaction Stereoselectivity
Source PublicationELECTROPHORESIS
1999-07-01
Volume20Issue:9Pages:1869-1877
Indexed ByISTP ; SCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordPARTIAL-FILLING TECHNIQUE ; AFFINITY ELECTROKINETIC CHROMATOGRAPHY ; HUMAN ALPHA(1)-ACID GLYCOPROTEIN ; ZONE-ELECTROPHORESIS ; PROTEIN-BINDING ; STEREOSELECTIVE BINDING ; KETOPROFEN ENANTIOMERS ; FRONTAL ANALYSIS ; SOLVATING AGENT ; BASIC DRUG
AbstractThe separation of the enantiomers of three basic drugs, i.e., ofloxacin, propranolol and verapamil, was achieved by affinity capillary electrophoresis (ACE), with human serum albumin (HSA) and bovine serum albumin (BSA) as chiral selectors in phosphate buffer at pH 7.4. Ofloxacin was only separated in the presence of BSA, and verapamil only with HSA, while propranolol was separated with either HSA or BSA. The effects of protein concentration and column wall adsorption on the degree of separation were investigated. Two displacers, ketoprofen and warfarin, respectively, when added to the protein containing buffer, both showed significant effects on the separation behavior. From these data it was argued that verapamil may bind to HSA at both locations known, the warfarin binding site (I) and the ketoprofen binding site (II). While with BSA, binding of ofloxacin may also occur at site I, the preferential binding site for propanolol remains controversial. A drug-drug interaction between propranolol and ketoprofen due to opposite charges was concluded from the increase in migration time in BSA solution. The unbound concentration of verapamil enantiomers in solution in the presence of HSA, as estimated from CD-modified capillary zone electrophoresis, was triggered not only by the HSA concentration but also by the coadditive concentration.
Language英语
WOS IDWOS:000081688800011
Citation statistics
Cited Times:45[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/138652
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Tubingen, Inst Organ Chem, D-72074 Tubingen, Germany
Recommended Citation
GB/T 7714
Zhu, XF,Ding, YS,Lin, BC,et al. Study of enantioselective interactions between chiral drugs and serum albumin by capillary electrophoresis[J]. ELECTROPHORESIS,1999,20(9):1869-1877.
APA Zhu, XF,Ding, YS,Lin, BC,Jakob, A,&Koppenhoefer, B.(1999).Study of enantioselective interactions between chiral drugs and serum albumin by capillary electrophoresis.ELECTROPHORESIS,20(9),1869-1877.
MLA Zhu, XF,et al."Study of enantioselective interactions between chiral drugs and serum albumin by capillary electrophoresis".ELECTROPHORESIS 20.9(1999):1869-1877.
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