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题名: Molecular verification of the integration of Tripsacum dactyloides DNA into wheat genome through wide hybridization
作者: Qiu, JW;  Jin, DM;  Li, CY;  Jia, JH;  Ouyang, P;  Tai, W;  Wang, B;  Li, DW
关键词: bread wheat ;  Tripsacum dactyloides ;  wide hybridization ;  RAPD ;  RFLP
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2000-03-01
卷: 45, 期:6, 页:528-532
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
英文摘要: RAPD and RFLP analyses of double haploid lines which derived from hybridization between hexaploid wheat (Triticum aestivum L. 2n=42) and eastern gamagrass (Tripsacum dactyloides L. 2n=4x=72) are reported. Two of the 340 Operon primers have been screened, which stably amplified Tripsacum dactyloides (male parent) specific bands in the double haploid lines. These results confirm the fact that Tripsacum dactyloides DNA has been integrated into wheat genome by sexual hybridization at molecular level. This idea has been further testified by RFLP analysis. Application and potentials of transferring Tripsacum dactyloides DNA into wheat genome by sexual hybridization in wheat breeding are discussed.
关键词[WOS]: CROSSES ;  FERTILIZATION
语种: 英语
WOS记录号: WOS:000086026100011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138678
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Genet Inst, Biotechnol Lab, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Genet Inst, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China
3.Univ Maryland, Inst Global Chinese Affairs, College Pk, MD 20742 USA

Recommended Citation:
Qiu, JW,Jin, DM,Li, CY,et al. Molecular verification of the integration of Tripsacum dactyloides DNA into wheat genome through wide hybridization[J]. CHINESE SCIENCE BULLETIN,2000,45(6):528-532.
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