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Molecular verification of the integration of Tripsacum dactyloides DNA into wheat genome through wide hybridization
Qiu, JW; Jin, DM; Li, CY; Jia, JH; Ouyang, P; Tai, W; Wang, B; Li, DW
关键词Bread Wheat Tripsacum Dactyloides Wide Hybridization Rapd Rflp
刊名CHINESE SCIENCE BULLETIN
2000-03-01
45期:6页:528-532
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]CROSSES ; FERTILIZATION
英文摘要RAPD and RFLP analyses of double haploid lines which derived from hybridization between hexaploid wheat (Triticum aestivum L. 2n=42) and eastern gamagrass (Tripsacum dactyloides L. 2n=4x=72) are reported. Two of the 340 Operon primers have been screened, which stably amplified Tripsacum dactyloides (male parent) specific bands in the double haploid lines. These results confirm the fact that Tripsacum dactyloides DNA has been integrated into wheat genome by sexual hybridization at molecular level. This idea has been further testified by RFLP analysis. Application and potentials of transferring Tripsacum dactyloides DNA into wheat genome by sexual hybridization in wheat breeding are discussed.
语种英语
WOS记录号WOS:000086026100011
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/138678
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Genet Inst, Biotechnol Lab, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Genet Inst, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China
3.Univ Maryland, Inst Global Chinese Affairs, College Pk, MD 20742 USA
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Qiu, JW,Jin, DM,Li, CY,et al. Molecular verification of the integration of Tripsacum dactyloides DNA into wheat genome through wide hybridization[J]. CHINESE SCIENCE BULLETIN,2000,45(6):528-532.
APA Qiu, JW.,Jin, DM.,Li, CY.,Jia, JH.,Ouyang, P.,...&Li, DW.(2000).Molecular verification of the integration of Tripsacum dactyloides DNA into wheat genome through wide hybridization.CHINESE SCIENCE BULLETIN,45(6),528-532.
MLA Qiu, JW,et al."Molecular verification of the integration of Tripsacum dactyloides DNA into wheat genome through wide hybridization".CHINESE SCIENCE BULLETIN 45.6(2000):528-532.
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