DICP OpenIR
Molecular verification of the integration of Tripsacum dactyloides DNA into wheat genome through wide hybridization
Qiu, JW; Jin, DM; Li, CY; Jia, JH; Ouyang, P; Tai, W; Wang, B; Li, DW
KeywordBread Wheat Tripsacum Dactyloides Wide Hybridization Rapd Rflp
Source PublicationCHINESE SCIENCE BULLETIN
2000-03-01
Volume45Issue:6Pages:528-532
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology
WOS SubjectMultidisciplinary Sciences
WOS Research AreaScience & Technology - Other Topics
WOS KeywordCROSSES ; FERTILIZATION
AbstractRAPD and RFLP analyses of double haploid lines which derived from hybridization between hexaploid wheat (Triticum aestivum L. 2n=42) and eastern gamagrass (Tripsacum dactyloides L. 2n=4x=72) are reported. Two of the 340 Operon primers have been screened, which stably amplified Tripsacum dactyloides (male parent) specific bands in the double haploid lines. These results confirm the fact that Tripsacum dactyloides DNA has been integrated into wheat genome by sexual hybridization at molecular level. This idea has been further testified by RFLP analysis. Application and potentials of transferring Tripsacum dactyloides DNA into wheat genome by sexual hybridization in wheat breeding are discussed.
Language英语
WOS IDWOS:000086026100011
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/138678
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Genet Inst, Biotechnol Lab, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Genet Inst, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China
3.Univ Maryland, Inst Global Chinese Affairs, College Pk, MD 20742 USA
Recommended Citation
GB/T 7714
Qiu, JW,Jin, DM,Li, CY,et al. Molecular verification of the integration of Tripsacum dactyloides DNA into wheat genome through wide hybridization[J]. CHINESE SCIENCE BULLETIN,2000,45(6):528-532.
APA Qiu, JW.,Jin, DM.,Li, CY.,Jia, JH.,Ouyang, P.,...&Li, DW.(2000).Molecular verification of the integration of Tripsacum dactyloides DNA into wheat genome through wide hybridization.CHINESE SCIENCE BULLETIN,45(6),528-532.
MLA Qiu, JW,et al."Molecular verification of the integration of Tripsacum dactyloides DNA into wheat genome through wide hybridization".CHINESE SCIENCE BULLETIN 45.6(2000):528-532.
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