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Comparative separation of biologically active components in Rhizoma chuanxiong by affinity chromatography with alpha(1)-acid glycoprotein and human serum albumin as stationary phases
Wang, H; Zou, H; Ni, J; Guo, B
关键词Column Liquid Chromatography Alpha(1)-acid Glycoprotein Human Serum Albumin Rhizoma Chuanxiong
刊名CHROMATOGRAPHIA
2000-10-01
52期:7-8页:459-464
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]LIGAND-BINDING
英文摘要Affinity chromatography with alpha (1)-acid glycoprotein (AGP) and human serum albumin (HSA) stationary phases was applied to screen and analyze the biologically active components of Rhizoma chuanxiong. Five major peaks and a number of small peaks were resolved based on their affinity for AGP and HSA, respectively and three of them, identified as ferulic acid, chuanxiongzine and ligustilide via standard compounds, are regarded as effective components. The effects of acetonitrile concentration, pH, inorganic salt concentration and temperature on the retention behaviors of five major components on the two stationary phases were also investigated. It was observed that hydrophobicity is the major contributor to retention on both stationary phases, and ferulic acid has a weak electrostatic interaction with HSA. It demonstrated that the chromatograms of Rhizoma chuanxiong on the two stationary phases have concise and different fingerprinting characteristics. The amount of ferulic acid, chuanxiongzine and ligustilide in Rhizoma chuanxiong determined using the AGP column are as much as 0.064%, 0.021% and 2.00% by weight.
语种英语
WOS记录号WOS:000165295100014
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被引频次:24[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/138682
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
2.Cleveland State Univ, Dept Chem, Cleveland, OH 44115 USA
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GB/T 7714
Wang, H,Zou, H,Ni, J,et al. Comparative separation of biologically active components in Rhizoma chuanxiong by affinity chromatography with alpha(1)-acid glycoprotein and human serum albumin as stationary phases[J]. CHROMATOGRAPHIA,2000,52(7-8):459-464.
APA Wang, H,Zou, H,Ni, J,&Guo, B.(2000).Comparative separation of biologically active components in Rhizoma chuanxiong by affinity chromatography with alpha(1)-acid glycoprotein and human serum albumin as stationary phases.CHROMATOGRAPHIA,52(7-8),459-464.
MLA Wang, H,et al."Comparative separation of biologically active components in Rhizoma chuanxiong by affinity chromatography with alpha(1)-acid glycoprotein and human serum albumin as stationary phases".CHROMATOGRAPHIA 52.7-8(2000):459-464.
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