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题名: Application of capillary electrophoresis in clinical chemistry: the clinical value of urinary modified nucleosides
作者: Liebich, HM;  Lehmann, R;  Xu, G;  Wahl, HG;  Haring, HU
关键词: nucleosides
刊名: JOURNAL OF CHROMATOGRAPHY B
发表日期: 2000-08-04
卷: 745, 期:1, 页:189-196
收录类别: ISTP ;  SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Urinary modified nucleosides were determined by capillary electrophoresis using a 300 mM SDS-25 mM sodium tetraborate-50 mM sodium dihydrogenphosphate buffer. The nucleosides were extracted from urine by phenylboronate affinity gel chromatography. In cancer patients the levels of the modified nucleosides are generally elevated. By an artificial neural network method breast cancer patients were differentiated from normal individuals, which indicates that the modified nucleosides could be of clinical value as tumor markers. (C) 2000 Elsevier Science B.V. All rights reserved.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  TRANSFER-RNA MODIFICATION ;  SERUM PSEUDOURIDINE ;  DEGRADATION RATES ;  TURNOVER ;  MARKER ;  RIBONUCLEOSIDE ;  SEPARATION ;  DIAGNOSIS ;  CANCER
语种: 英语
WOS记录号: WOS:000088810600015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138694
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Univ Tubingen, Med Klin, Abt 4, D-72076 Tubingen, Germany
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Liebich, HM,Lehmann, R,Xu, G,et al. Application of capillary electrophoresis in clinical chemistry: the clinical value of urinary modified nucleosides[J]. JOURNAL OF CHROMATOGRAPHY B,2000,745(1):189-196.
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