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题名: Purification and identification of PEGlated hemoglobin, a potential blood substitute, by chromatography and capillary electrophoresis
作者: Li, W;  Zhang, D;  Lin, B;  Su, Z
关键词: column liquid chromatography ;  size-exclusion chromatography ;  capillary electrophoresis ;  PEGlated hemoglobin
刊名: CHROMATOGRAPHIA
发表日期: 2000-10-01
卷: 52, 期:7-8, 页:451-454
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Bovine hemoglobin (Hb) has been chemically modified, by reaction of its lysine residues with the active ester of poly(ethylene glycol) (PEG, M-w = 5000), to produce a potential blood substitute for human therapy. Covalent attachment of PEG chains to the protein produced a heterogeneous mixture of PEGlated Hb (PEG-Hb). It is difficult but necessary to remove unmodified and poorly modified Hb from the mixture. This paper describes the use of cation-exchange chromatography (IEC), in flow-through mode, and size-exclusion chromatography (SEC) for purification of the PEG-Hb mixture. The highly modified Hb flowed through the IEC column in the loading buffer without adsorption by the chromatographic medium. SEC was then used for further purification. These two steps were suitable for pilot-scale preparation or for analytical chromatography. The purified product was assessed by high-performance capillary electrophoresis (HPCE), which was also used to optimize the chromatographic parameters.
关键词[WOS]: POLYETHYLENE-GLYCOL ;  SUPEROXIDE-DISMUTASE
语种: 英语
WOS记录号: WOS:000165295100012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138718
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
Li, W,Zhang, D,Lin, B,et al. Purification and identification of PEGlated hemoglobin, a potential blood substitute, by chromatography and capillary electrophoresis[J]. CHROMATOGRAPHIA,2000,52(7-8):451-454.
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