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Purification and identification of PEGlated hemoglobin, a potential blood substitute, by chromatography and capillary electrophoresis
Li, W; Zhang, D; Lin, B; Su, Z
关键词Column Liquid Chromatography Size-exclusion Chromatography Capillary Electrophoresis Peglated Hemoglobin
刊名CHROMATOGRAPHIA
2000-10-01
52期:7-8页:451-454
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]POLYETHYLENE-GLYCOL ; SUPEROXIDE-DISMUTASE
英文摘要Bovine hemoglobin (Hb) has been chemically modified, by reaction of its lysine residues with the active ester of poly(ethylene glycol) (PEG, M-w = 5000), to produce a potential blood substitute for human therapy. Covalent attachment of PEG chains to the protein produced a heterogeneous mixture of PEGlated Hb (PEG-Hb). It is difficult but necessary to remove unmodified and poorly modified Hb from the mixture. This paper describes the use of cation-exchange chromatography (IEC), in flow-through mode, and size-exclusion chromatography (SEC) for purification of the PEG-Hb mixture. The highly modified Hb flowed through the IEC column in the loading buffer without adsorption by the chromatographic medium. SEC was then used for further purification. These two steps were suitable for pilot-scale preparation or for analytical chromatography. The purified product was assessed by high-performance capillary electrophoresis (HPCE), which was also used to optimize the chromatographic parameters.
语种英语
WOS记录号WOS:000165295100012
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被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/138718
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
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GB/T 7714
Li, W,Zhang, D,Lin, B,et al. Purification and identification of PEGlated hemoglobin, a potential blood substitute, by chromatography and capillary electrophoresis[J]. CHROMATOGRAPHIA,2000,52(7-8):451-454.
APA Li, W,Zhang, D,Lin, B,&Su, Z.(2000).Purification and identification of PEGlated hemoglobin, a potential blood substitute, by chromatography and capillary electrophoresis.CHROMATOGRAPHIA,52(7-8),451-454.
MLA Li, W,et al."Purification and identification of PEGlated hemoglobin, a potential blood substitute, by chromatography and capillary electrophoresis".CHROMATOGRAPHIA 52.7-8(2000):451-454.
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