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New affinity nylon membrane used for adsorption of gamma-globulin
Gan, HY; Shang, ZH; Wang, JD
关键词Membranes Affinity Membranes Stacking Globulin Proteins
刊名JOURNAL OF CHROMATOGRAPHY A
2000-01-21
867期:1-2页:161-168
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]AMINO-ACIDS ; PROTEINS ; REMOVAL ; CHROMATOGRAPHY ; SEPARATION ; ENDOTOXIN ; BINDING ; LIGANDS
英文摘要Microporous polyamide membranes were first modified by acid hydrolysis and subsequently bound with hydroxyethylcellulose to amplify reactive groups and reduce nonspecific interactions with proteins. Then 1,6-diaminohexane as space arm and phenylalanine as ligand were immobilized onto the nylon membranes by s-triazine trichloride activation. Affinity membranes thus obtained were set in a stack and used to adsorb gamma-globulin. The adsorption capacity (q(m)) of the affinity membrane is 53 mu g gamma-globulin per m(2) membrane and the desorption constant (K-d) is 2.35 . 10(-6) mel/l. The effects of feed, washing and elution rates on adsorption and desorption behavior were investigated. The results showed that affinity purification through these membranes could not be operated at very high flow-rates. A stack of 20 membranes with 47 mm diameter can adsorb 7.8 mg gamma-globulin with a purity of 91.6% from 4 mi of human plasma in a single-pass mode. (C) 2000 Elsevier Science B.V. All rights reserved.
语种英语
WOS记录号WOS:000084838400012
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被引频次:41[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/138724
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China
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Gan, HY,Shang, ZH,Wang, JD. New affinity nylon membrane used for adsorption of gamma-globulin[J]. JOURNAL OF CHROMATOGRAPHY A,2000,867(1-2):161-168.
APA Gan, HY,Shang, ZH,&Wang, JD.(2000).New affinity nylon membrane used for adsorption of gamma-globulin.JOURNAL OF CHROMATOGRAPHY A,867(1-2),161-168.
MLA Gan, HY,et al."New affinity nylon membrane used for adsorption of gamma-globulin".JOURNAL OF CHROMATOGRAPHY A 867.1-2(2000):161-168.
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