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题名: New affinity nylon membrane used for adsorption of gamma-globulin
作者: Gan, HY;  Shang, ZH;  Wang, JD
关键词: membranes ;  affinity membranes ;  stacking ;  globulin ;  proteins
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2000-01-21
卷: 867, 期:1-2, 页:161-168
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Microporous polyamide membranes were first modified by acid hydrolysis and subsequently bound with hydroxyethylcellulose to amplify reactive groups and reduce nonspecific interactions with proteins. Then 1,6-diaminohexane as space arm and phenylalanine as ligand were immobilized onto the nylon membranes by s-triazine trichloride activation. Affinity membranes thus obtained were set in a stack and used to adsorb gamma-globulin. The adsorption capacity (q(m)) of the affinity membrane is 53 mu g gamma-globulin per m(2) membrane and the desorption constant (K-d) is 2.35 . 10(-6) mel/l. The effects of feed, washing and elution rates on adsorption and desorption behavior were investigated. The results showed that affinity purification through these membranes could not be operated at very high flow-rates. A stack of 20 membranes with 47 mm diameter can adsorb 7.8 mg gamma-globulin with a purity of 91.6% from 4 mi of human plasma in a single-pass mode. (C) 2000 Elsevier Science B.V. All rights reserved.
关键词[WOS]: AMINO-ACIDS ;  PROTEINS ;  REMOVAL ;  CHROMATOGRAPHY ;  SEPARATION ;  ENDOTOXIN ;  BINDING ;  LIGANDS
语种: 英语
WOS记录号: WOS:000084838400012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138724
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China

Recommended Citation:
Gan, HY,Shang, ZH,Wang, JD. New affinity nylon membrane used for adsorption of gamma-globulin[J]. JOURNAL OF CHROMATOGRAPHY A,2000,867(1-2):161-168.
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