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题名: Thermodynamics of porphyin binding to human serum albumin using affinity capillary electrophoresis
作者: Ding, YS;  Lin, BC;  Huie, CW
关键词: affinity capillary electrophoresis ;  porphyrins ;  human serum albumin ;  binding constants ;  thermodynamic parameters
刊名: CHROMATOGRAPHIA
发表日期: 2000-09-01
卷: 52, 期:5-6, 页:367-370
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The interaction thermodynamics of heptacarboxylporphyrin (HCP) and protoporhyrin (PP) with human serum albumin (HSA) was studied by affinity capillary electrophoresis (ACE) over the temperature range of 25 - 50 degrees C, where HCP PP bound to HSA via 1:1 molecular association. The binding equilibrium constants (pH 7.4, phosphate buffer) for the binding of HCP with HSA were found to decrease wi ih an increase in temperature, whereas the binding constants of the PP/HSA system appeared to be independent of temperature changes over the range studied. The van't Hoff relationship (25 - 50 degrees C) was found to be linear for the interaction of either HCP or PP with HSA. However, the interaction thermodynamics for both of these porphyrins with HSA were found to be quite different. in particular the interaction of HCP (a hydrophilic porphyrin) with HSA appeared to be based on an enthalpy-driven process, whereas the binding between PP (a hydrophobic porphyrin) and HSA driven by a favorable change in entropy. The ability of using ACE to evaluate the interaction thermodynamics of serum proteins (e.g, HSA) with ligands (e.g., porphyrins and related compounds) should aid in the development of new and more effective photosensitizers in the photodynamic therapy of cancer.
语种: 英语
WOS记录号: WOS:000089660600019
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138769
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Hong Kong Baptist Univ, Dept Chem, Kowloon, Hong Kong, Peoples R China

Recommended Citation:
Ding, YS,Lin, BC,Huie, CW. Thermodynamics of porphyin binding to human serum albumin using affinity capillary electrophoresis[J]. CHROMATOGRAPHIA,2000,52(5-6):367-370.
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