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On-line characterization of the activity and reaction kinetics of immobilized enzyme by high-performance frontal analysis
Jiang, HH; Zou, HF; Wang, HL; Ni, JY; Zhang, Q; Zhang, YK
KeywordImmobilized Trypsin Reactor Glycidyl Methacrylate-modified Membrane Reaction Kinetics Frontal Analysis Enzymes
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2000-12-15
Volume903Issue:1-2Pages:77-84
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordAFFINITY-CHROMATOGRAPHY ; GLUCOSE-OXIDASE ; PURIFICATION ; MEMBRANES ; RETENTION ; TRYPSIN ; SPACER
AbstractA microreactor by immobilized trypsin on the activated glycidyl methacrylate-modified cellulose membrane packed column was constructed, Immobilized trypsin mirrored the properties of the free enzyme and showed high stability. A novel method to characterize the activity and reaction kinetics of the immobilized enzyme has been developed based on the frontal analysis of enzymatic reaction products, which was performed by the on-line monitoring of the absorption at 410 nm of p-nitroaniline from the hydrolysis of N-alpha -benzoyl-DL-arginine-p-nitroanilide (BAPNA). The hydrolytic activity of the immobilized enzyme was 55.6% of free trypsin. The apparent Michaelis-Menten kinetics constant (K-m) and V-max values measured by the frontal analysis method were, respectively, 0.12 mM and 0.079 mM min(-1) mg enzyme(-1). The former is very close to that observed by the static and off-line detection methods, but the latter is about 15% higher than that of the static method. Inhibition of the immobilized trypsin by addition of benzamidine into substrate solution has been studied by the frontal analysis method. The apparent Michaelis-Menten constant of BAPNA (K-m), the inhibition constant of benzamidine (K-m) and V-max were determined. It was indicated that the interaction of BAPNA and benzamidine with trypsin is competitive, the K-m value was affected but the V-max was unaffected by the benzamidine concentration. (C) 2000 Elsevier Science B.V. All rights reserved.
Language英语
WOS IDWOS:000165977800008
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/138773
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromat R&A Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Jiang, HH,Zou, HF,Wang, HL,et al. On-line characterization of the activity and reaction kinetics of immobilized enzyme by high-performance frontal analysis[J]. JOURNAL OF CHROMATOGRAPHY A,2000,903(1-2):77-84.
APA Jiang, HH,Zou, HF,Wang, HL,Ni, JY,Zhang, Q,&Zhang, YK.(2000).On-line characterization of the activity and reaction kinetics of immobilized enzyme by high-performance frontal analysis.JOURNAL OF CHROMATOGRAPHY A,903(1-2),77-84.
MLA Jiang, HH,et al."On-line characterization of the activity and reaction kinetics of immobilized enzyme by high-performance frontal analysis".JOURNAL OF CHROMATOGRAPHY A 903.1-2(2000):77-84.
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