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Combination of MALDI-TOF mass spectrometry with immobilized enzyme microreactor for peptide mapping
Jiang, HH; Zou, HF; Wang, HL; Zhang, Q; Ni, JY; Zhang, QC; Guo, Z; Chen, XM
关键词Immobilized Enzyme Microreactor Glycidyl Methacrylate (Gma)-modified Cellulose Membrane Maldi-tof Ms Peptide Mapping
刊名SCIENCE IN CHINA SERIES B-CHEMISTRY
2000-12-01
43期:6页:625-633
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]CHROMATOGRAPHY ; PROTEINS
英文摘要Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been combined with immobilized enzyme microreactor for the rapid, sensitive, and accurate tryptic mapping of protein and polypeptides. The technique utilizes the trypsin microreactor by immobilized enzyme on the glycidyl methacrylate (GMA)-modified cellulose membrane. The membrane microreactor was used for the tryptic mapping of cytochrome C and the results were compared with those obtained by using free trypsin. A significant increase in the overall sensitivity of the process was observed using the membrane microreactor, as well as the elimination of background signals due to the autolysis of the trypsin. Further, membrane microreactor digestions were found to be rapid and convenient.
语种英语
WOS记录号WOS:000166664000008
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/138776
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
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GB/T 7714
Jiang, HH,Zou, HF,Wang, HL,et al. Combination of MALDI-TOF mass spectrometry with immobilized enzyme microreactor for peptide mapping[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2000,43(6):625-633.
APA Jiang, HH.,Zou, HF.,Wang, HL.,Zhang, Q.,Ni, JY.,...&Chen, XM.(2000).Combination of MALDI-TOF mass spectrometry with immobilized enzyme microreactor for peptide mapping.SCIENCE IN CHINA SERIES B-CHEMISTRY,43(6),625-633.
MLA Jiang, HH,et al."Combination of MALDI-TOF mass spectrometry with immobilized enzyme microreactor for peptide mapping".SCIENCE IN CHINA SERIES B-CHEMISTRY 43.6(2000):625-633.
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