DICP OpenIR
Combination of MALDI-TOF mass spectrometry with immobilized enzyme microreactor for peptide mapping
Jiang, HH; Zou, HF; Wang, HL; Zhang, Q; Ni, JY; Zhang, QC; Guo, Z; Chen, XM
KeywordImmobilized Enzyme Microreactor Glycidyl Methacrylate (Gma)-modified Cellulose Membrane Maldi-tof Ms Peptide Mapping
Source PublicationSCIENCE IN CHINA SERIES B-CHEMISTRY
2000-12-01
Volume43Issue:6Pages:625-633
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordCHROMATOGRAPHY ; PROTEINS
AbstractMatrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been combined with immobilized enzyme microreactor for the rapid, sensitive, and accurate tryptic mapping of protein and polypeptides. The technique utilizes the trypsin microreactor by immobilized enzyme on the glycidyl methacrylate (GMA)-modified cellulose membrane. The membrane microreactor was used for the tryptic mapping of cytochrome C and the results were compared with those obtained by using free trypsin. A significant increase in the overall sensitivity of the process was observed using the membrane microreactor, as well as the elimination of background signals due to the autolysis of the trypsin. Further, membrane microreactor digestions were found to be rapid and convenient.
Language英语
WOS IDWOS:000166664000008
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/138776
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Jiang, HH,Zou, HF,Wang, HL,et al. Combination of MALDI-TOF mass spectrometry with immobilized enzyme microreactor for peptide mapping[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2000,43(6):625-633.
APA Jiang, HH.,Zou, HF.,Wang, HL.,Zhang, Q.,Ni, JY.,...&Chen, XM.(2000).Combination of MALDI-TOF mass spectrometry with immobilized enzyme microreactor for peptide mapping.SCIENCE IN CHINA SERIES B-CHEMISTRY,43(6),625-633.
MLA Jiang, HH,et al."Combination of MALDI-TOF mass spectrometry with immobilized enzyme microreactor for peptide mapping".SCIENCE IN CHINA SERIES B-CHEMISTRY 43.6(2000):625-633.
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