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Study on the direct interaction between aminoacylase and Cu(II) ions by spectroscopic analysis
Zheng, XF; Wang, JY; Wang, Y; An, LJ; Gai, HW; Hu, J
关键词Esr Spectroscopy Alkaline Phosphatase Cu(Ii) Ions Interaction
刊名SPECTROSCOPY AND SPECTRAL ANALYSIS
2001-12-01
21期:6页:804-806
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Technology
类目[WOS]Spectroscopy
研究领域[WOS]Spectroscopy
英文摘要In this paper, the method of reconstitution was used to investigate the interaction between metalloenzymes (containing Zn ( H)) and metal ions. Electron paramagnetic resonance(EPR) visible spectrum (Vis) and enzyme activity assay have been employed to study the direct interactions between aminoacylase (ACY) and Cu( 11) ions added in aqueous solution. The results show that a dynamic equilibrium exists between the Zn( H) in the active site of native enzymes and the added Cu( 11) the added Cu(II) partly replaces the Zn( U), forming Cu( H)-enzyme derivatives. As a result, the activity of the native enzymes is influenced. In addition, the influences of pH value on this kind of interaction have also been investigated, and the results demonstrate that the decrease in the intensity of the Cu ( 11) EPR signal and the change of place signal in Vis were observed as increase of pH value. These results suggest that the derivative of Cu( It)-ACY exists in solution with two different conformations, and this two conformations exchanged each other depending on pH.
语种英语
WOS记录号WOS:000173061200020
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/138839
专题中国科学院大连化学物理研究所
作者单位1.Dalian Univ, Coll Bioengn, Dalian 116622, Peoples R China
2.Dalian Univ Technol, Dept Bioengn, Dalian 116012, Peoples R China
3.Acad Sinica, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
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GB/T 7714
Zheng, XF,Wang, JY,Wang, Y,et al. Study on the direct interaction between aminoacylase and Cu(II) ions by spectroscopic analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2001,21(6):804-806.
APA Zheng, XF,Wang, JY,Wang, Y,An, LJ,Gai, HW,&Hu, J.(2001).Study on the direct interaction between aminoacylase and Cu(II) ions by spectroscopic analysis.SPECTROSCOPY AND SPECTRAL ANALYSIS,21(6),804-806.
MLA Zheng, XF,et al."Study on the direct interaction between aminoacylase and Cu(II) ions by spectroscopic analysis".SPECTROSCOPY AND SPECTRAL ANALYSIS 21.6(2001):804-806.
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