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题名: Study on the direct interaction between aminoacylase and Cu(II) ions by spectroscopic analysis
作者: Zheng, XF;  Wang, JY;  Wang, Y;  An, LJ;  Gai, HW;  Hu, J
关键词: ESR spectroscopy ;  alkaline phosphatase ;  Cu(II) ions ;  interaction
刊名: SPECTROSCOPY AND SPECTRAL ANALYSIS
发表日期: 2001-12-01
卷: 21, 期:6, 页:804-806
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Spectroscopy
研究领域[WOS]: Spectroscopy
英文摘要: In this paper, the method of reconstitution was used to investigate the interaction between metalloenzymes (containing Zn ( H)) and metal ions. Electron paramagnetic resonance(EPR) visible spectrum (Vis) and enzyme activity assay have been employed to study the direct interactions between aminoacylase (ACY) and Cu( 11) ions added in aqueous solution. The results show that a dynamic equilibrium exists between the Zn( H) in the active site of native enzymes and the added Cu( 11) the added Cu(II) partly replaces the Zn( U), forming Cu( H)-enzyme derivatives. As a result, the activity of the native enzymes is influenced. In addition, the influences of pH value on this kind of interaction have also been investigated, and the results demonstrate that the decrease in the intensity of the Cu ( 11) EPR signal and the change of place signal in Vis were observed as increase of pH value. These results suggest that the derivative of Cu( It)-ACY exists in solution with two different conformations, and this two conformations exchanged each other depending on pH.
语种: 英语
WOS记录号: WOS:000173061200020
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138839
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Univ, Coll Bioengn, Dalian 116622, Peoples R China
2.Dalian Univ Technol, Dept Bioengn, Dalian 116012, Peoples R China
3.Acad Sinica, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Zheng, XF,Wang, JY,Wang, Y,et al. Study on the direct interaction between aminoacylase and Cu(II) ions by spectroscopic analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2001,21(6):804-806.
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