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Effect of the pore sizes of nylon affinity membranes on gamma-globulin adsorption
Gan, HY; Shang, ZH; Wang, JD; Liu, XL
KeywordAffinity Nylon Membranes Pore-size Effects Hydroxyethylcellulose Gamma-globulin Adsorption Human Plasma
Source PublicationCHROMATOGRAPHIA
2001-04-01
Volume53Issue:7-8Pages:450-453
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordCHROMATOGRAPHY ; PERFORMANCE ; PROTEINS
AbstractL-Phenylalanine was immobilized on nylon membranes with two pore diameters (0.45 mum and 1 mum), by activation with 1,4-butanediol diglycidyl ether, and the effect of pore size on the affinity adsorption of gamma -globulin studied by batch and kinetic methods. Experiment shows that adsorption on both affinity membranes obeys the Freundlich model. The accessible pore volume for adsorption of proteins on the membrane with 0.45 mum diameter pores is less than for that with 1 mum diameter pores. The adsorption capacity of affinity membranes with 1 mum diameter pores is 2.5-fold that of membranes with 0.45 mum diameter pores. Feed-rate has a larger effect on affinity adsorption on the membrane with 0.45 mum diameter pores than on that with 1 mum diameter pores. Small pores on the affinity membrane do not cause broadening of the elution peak. It is concluded that affinity interaction and separation occur mainly in the large pores, and small pore size does not favor improvement of adsorption capacity, gamma -Globulin 85.1% pure can be obtained in one step from human plasma by use of the affinity membrane with 0.45 mum diameter pores.
Language英语
WOS IDWOS:000168406700018
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/138852
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Gan, HY,Shang, ZH,Wang, JD,et al. Effect of the pore sizes of nylon affinity membranes on gamma-globulin adsorption[J]. CHROMATOGRAPHIA,2001,53(7-8):450-453.
APA Gan, HY,Shang, ZH,Wang, JD,&Liu, XL.(2001).Effect of the pore sizes of nylon affinity membranes on gamma-globulin adsorption.CHROMATOGRAPHIA,53(7-8),450-453.
MLA Gan, HY,et al."Effect of the pore sizes of nylon affinity membranes on gamma-globulin adsorption".CHROMATOGRAPHIA 53.7-8(2001):450-453.
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