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Effect of the pore sizes of nylon affinity membranes on gamma-globulin adsorption
Gan, HY; Shang, ZH; Wang, JD; Liu, XL
关键词Affinity Nylon Membranes Pore-size Effects Hydroxyethylcellulose Gamma-globulin Adsorption Human Plasma
刊名CHROMATOGRAPHIA
2001-04-01
53期:7-8页:450-453
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]CHROMATOGRAPHY ; PERFORMANCE ; PROTEINS
英文摘要L-Phenylalanine was immobilized on nylon membranes with two pore diameters (0.45 mum and 1 mum), by activation with 1,4-butanediol diglycidyl ether, and the effect of pore size on the affinity adsorption of gamma -globulin studied by batch and kinetic methods. Experiment shows that adsorption on both affinity membranes obeys the Freundlich model. The accessible pore volume for adsorption of proteins on the membrane with 0.45 mum diameter pores is less than for that with 1 mum diameter pores. The adsorption capacity of affinity membranes with 1 mum diameter pores is 2.5-fold that of membranes with 0.45 mum diameter pores. Feed-rate has a larger effect on affinity adsorption on the membrane with 0.45 mum diameter pores than on that with 1 mum diameter pores. Small pores on the affinity membrane do not cause broadening of the elution peak. It is concluded that affinity interaction and separation occur mainly in the large pores, and small pore size does not favor improvement of adsorption capacity, gamma -Globulin 85.1% pure can be obtained in one step from human plasma by use of the affinity membrane with 0.45 mum diameter pores.
语种英语
WOS记录号WOS:000168406700018
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/138852
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
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GB/T 7714
Gan, HY,Shang, ZH,Wang, JD,et al. Effect of the pore sizes of nylon affinity membranes on gamma-globulin adsorption[J]. CHROMATOGRAPHIA,2001,53(7-8):450-453.
APA Gan, HY,Shang, ZH,Wang, JD,&Liu, XL.(2001).Effect of the pore sizes of nylon affinity membranes on gamma-globulin adsorption.CHROMATOGRAPHIA,53(7-8),450-453.
MLA Gan, HY,et al."Effect of the pore sizes of nylon affinity membranes on gamma-globulin adsorption".CHROMATOGRAPHIA 53.7-8(2001):450-453.
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