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题名: Effect of the pore sizes of nylon affinity membranes on gamma-globulin adsorption
作者: Gan, HY;  Shang, ZH;  Wang, JD;  Liu, XL
关键词: affinity nylon membranes ;  pore-size effects ;  hydroxyethylcellulose ;  gamma-globulin adsorption ;  human plasma
刊名: CHROMATOGRAPHIA
发表日期: 2001-04-01
卷: 53, 期:7-8, 页:450-453
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: L-Phenylalanine was immobilized on nylon membranes with two pore diameters (0.45 mum and 1 mum), by activation with 1,4-butanediol diglycidyl ether, and the effect of pore size on the affinity adsorption of gamma -globulin studied by batch and kinetic methods. Experiment shows that adsorption on both affinity membranes obeys the Freundlich model. The accessible pore volume for adsorption of proteins on the membrane with 0.45 mum diameter pores is less than for that with 1 mum diameter pores. The adsorption capacity of affinity membranes with 1 mum diameter pores is 2.5-fold that of membranes with 0.45 mum diameter pores. Feed-rate has a larger effect on affinity adsorption on the membrane with 0.45 mum diameter pores than on that with 1 mum diameter pores. Small pores on the affinity membrane do not cause broadening of the elution peak. It is concluded that affinity interaction and separation occur mainly in the large pores, and small pore size does not favor improvement of adsorption capacity, gamma -Globulin 85.1% pure can be obtained in one step from human plasma by use of the affinity membrane with 0.45 mum diameter pores.
关键词[WOS]: CHROMATOGRAPHY ;  PERFORMANCE ;  PROTEINS
语种: 英语
WOS记录号: WOS:000168406700018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138852
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China

Recommended Citation:
Gan, HY,Shang, ZH,Wang, JD,et al. Effect of the pore sizes of nylon affinity membranes on gamma-globulin adsorption[J]. CHROMATOGRAPHIA,2001,53(7-8):450-453.
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