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Separation of acidic and neutral compounds by strong anion-exchange capillary electrochromatography dynamically modified with sodium dodecylsulfate
Ye, ML; Zou, HF; Lei, ZD; Wu, RN; Liu, Z; Ni, JY
关键词Capillary Electrochromatography Strong Anion-exchange Stationary Phase Dynamic Modification Sds
刊名CHROMATOGRAPHIA
2001-04-01
53期:7-8页:425-430
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]MODIFIED CATIONIC SURFACTANT ; REVERSED-PHASE ; SILICA ; COLUMN ; ELECTROPHORESIS ; PEPTIDES
英文摘要Acidic and neutral compounds have been separated by strong anion-exchange capillary electrochromatography (SAXCEC) dynamically modified by addition of sodium dodecylsulfate (SDS). It was found that separation of neutral solutes by SAXCEC without addition of SDS is difficult because of the weak interaction of the solutes with the hydrophilic packing surface. The hydrophobicity of the packing surface increases on addition of SDS to the mobile phase, however, and the capacity of separating neutral solutes increases. Acidic solutes are retained, mainly because of the ion-exchange properties of this system. The influence of mobile phase composition, e.g. SDS concentration, ionic strength, and organic modifier fraction, on the retention of acidic and neutral solutes was investigated. Three acids and five neutral solutes were separated in only 5 min under optimized conditions, because the direction of the strong electroosmotic flow (EOF) was the some as that of electrophoretic migration of the acids under the conditions used. The repeatability of this system in terms of migration time relative standard deviation (RSD) is good - less than 0.48% for 10 consecutive runs of oil the solutes tested. Column efficiencies for acids were > 125000 plates m(-1); those for neutral solutes varied from 25 000 to 100 000 plates m(-1).
语种英语
WOS记录号WOS:000168406700013
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/138890
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
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GB/T 7714
Ye, ML,Zou, HF,Lei, ZD,et al. Separation of acidic and neutral compounds by strong anion-exchange capillary electrochromatography dynamically modified with sodium dodecylsulfate[J]. CHROMATOGRAPHIA,2001,53(7-8):425-430.
APA Ye, ML,Zou, HF,Lei, ZD,Wu, RN,Liu, Z,&Ni, JY.(2001).Separation of acidic and neutral compounds by strong anion-exchange capillary electrochromatography dynamically modified with sodium dodecylsulfate.CHROMATOGRAPHIA,53(7-8),425-430.
MLA Ye, ML,et al."Separation of acidic and neutral compounds by strong anion-exchange capillary electrochromatography dynamically modified with sodium dodecylsulfate".CHROMATOGRAPHIA 53.7-8(2001):425-430.
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