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Separation of acidic and neutral compounds by strong anion-exchange capillary electrochromatography dynamically modified with sodium dodecylsulfate
Ye, ML; Zou, HF; Lei, ZD; Wu, RN; Liu, Z; Ni, JY
KeywordCapillary Electrochromatography Strong Anion-exchange Stationary Phase Dynamic Modification Sds
Source PublicationCHROMATOGRAPHIA
2001-04-01
Volume53Issue:7-8Pages:425-430
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordMODIFIED CATIONIC SURFACTANT ; REVERSED-PHASE ; SILICA ; COLUMN ; ELECTROPHORESIS ; PEPTIDES
AbstractAcidic and neutral compounds have been separated by strong anion-exchange capillary electrochromatography (SAXCEC) dynamically modified by addition of sodium dodecylsulfate (SDS). It was found that separation of neutral solutes by SAXCEC without addition of SDS is difficult because of the weak interaction of the solutes with the hydrophilic packing surface. The hydrophobicity of the packing surface increases on addition of SDS to the mobile phase, however, and the capacity of separating neutral solutes increases. Acidic solutes are retained, mainly because of the ion-exchange properties of this system. The influence of mobile phase composition, e.g. SDS concentration, ionic strength, and organic modifier fraction, on the retention of acidic and neutral solutes was investigated. Three acids and five neutral solutes were separated in only 5 min under optimized conditions, because the direction of the strong electroosmotic flow (EOF) was the some as that of electrophoretic migration of the acids under the conditions used. The repeatability of this system in terms of migration time relative standard deviation (RSD) is good - less than 0.48% for 10 consecutive runs of oil the solutes tested. Column efficiencies for acids were > 125000 plates m(-1); those for neutral solutes varied from 25 000 to 100 000 plates m(-1).
Language英语
WOS IDWOS:000168406700013
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/138890
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Ye, ML,Zou, HF,Lei, ZD,et al. Separation of acidic and neutral compounds by strong anion-exchange capillary electrochromatography dynamically modified with sodium dodecylsulfate[J]. CHROMATOGRAPHIA,2001,53(7-8):425-430.
APA Ye, ML,Zou, HF,Lei, ZD,Wu, RN,Liu, Z,&Ni, JY.(2001).Separation of acidic and neutral compounds by strong anion-exchange capillary electrochromatography dynamically modified with sodium dodecylsulfate.CHROMATOGRAPHIA,53(7-8),425-430.
MLA Ye, ML,et al."Separation of acidic and neutral compounds by strong anion-exchange capillary electrochromatography dynamically modified with sodium dodecylsulfate".CHROMATOGRAPHIA 53.7-8(2001):425-430.
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