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题名: Separation of acidic and neutral compounds by strong anion-exchange capillary electrochromatography dynamically modified with sodium dodecylsulfate
作者: Ye, ML;  Zou, HF;  Lei, ZD;  Wu, RN;  Liu, Z;  Ni, JY
关键词: capillary electrochromatography ;  strong anion-exchange stationary phase ;  dynamic modification ;  SDS
刊名: CHROMATOGRAPHIA
发表日期: 2001-04-01
卷: 53, 期:7-8, 页:425-430
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Acidic and neutral compounds have been separated by strong anion-exchange capillary electrochromatography (SAXCEC) dynamically modified by addition of sodium dodecylsulfate (SDS). It was found that separation of neutral solutes by SAXCEC without addition of SDS is difficult because of the weak interaction of the solutes with the hydrophilic packing surface. The hydrophobicity of the packing surface increases on addition of SDS to the mobile phase, however, and the capacity of separating neutral solutes increases. Acidic solutes are retained, mainly because of the ion-exchange properties of this system. The influence of mobile phase composition, e.g. SDS concentration, ionic strength, and organic modifier fraction, on the retention of acidic and neutral solutes was investigated. Three acids and five neutral solutes were separated in only 5 min under optimized conditions, because the direction of the strong electroosmotic flow (EOF) was the some as that of electrophoretic migration of the acids under the conditions used. The repeatability of this system in terms of migration time relative standard deviation (RSD) is good - less than 0.48% for 10 consecutive runs of oil the solutes tested. Column efficiencies for acids were > 125000 plates m(-1); those for neutral solutes varied from 25 000 to 100 000 plates m(-1).
关键词[WOS]: MODIFIED CATIONIC SURFACTANT ;  REVERSED-PHASE ;  SILICA ;  COLUMN ;  ELECTROPHORESIS ;  PEPTIDES
语种: 英语
WOS记录号: WOS:000168406700013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138890
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Ye, ML,Zou, HF,Lei, ZD,et al. Separation of acidic and neutral compounds by strong anion-exchange capillary electrochromatography dynamically modified with sodium dodecylsulfate[J]. CHROMATOGRAPHIA,2001,53(7-8):425-430.
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