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题名: Chromatographic separation of proteins on metal immobilized iminodiacetic acid-bound molded monolithic rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate)
作者: Luo, QZ;  Zou, HF;  Xiao, XZ;  Guo, Z;  Kong, L;  Mao, XQ
关键词: monolithic rods ;  immobilized metal affinity chromatography ;  protein ;  iminodiacetic acid
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2001-08-17
卷: 926, 期:2, 页:255-264
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Continuous rod of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) was prepared by a free radical polymerization within the confines of a stainless-steel column. The epoxide groups of the rod were modified by a reaction with iminodiacetic acid (IDA) that affords the active site to form metal IDA chelates used for immobilized metal affinity chromatography (IMAC). The efficiency of coupling of IDA to the epoxide-contained matrix was studied as a function of reaction time and temperature. High-performance separation of proteins, based on immobilized different metals on the column, were described. The influence of pH on the adsorption capacity of bovine serum albumin on the CU2+-IDA continuous rod column was investigated in the range from 5.0 to 9.0. Purification of lysozyme from egg white and human serum albumin (HSA) on the commercially available HSA solution were performed on the naked IDA and Cu2+-IDA continuous rod columns, respectively; and the purity of the obtained fractions was detected by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry. (C) 2001 Elsevier Science B.V. All rights reserved.
关键词[WOS]: ION AFFINITY-CHROMATOGRAPHY ;  CATION-EXCHANGE CHROMATOGRAPHY ;  REVERSED-PHASE CHROMATOGRAPHY ;  RIGID POLYMER MONOLITHS ;  CAPILLARY ELECTROCHROMATOGRAPHY ;  STATIONARY-PHASE ;  POROUS PROPERTIES ;  ESCHERICHIA-COLI ;  CONTINUOUS BEDS ;  MEMBRANE
语种: 英语
WOS记录号: WOS:000170684200003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138900
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Acad Sinica, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Luo, QZ,Zou, HF,Xiao, XZ,et al. Chromatographic separation of proteins on metal immobilized iminodiacetic acid-bound molded monolithic rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate)[J]. JOURNAL OF CHROMATOGRAPHY A,2001,926(2):255-264.
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