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Chromatographic separation of proteins on metal immobilized iminodiacetic acid-bound molded monolithic rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate)
Luo, QZ; Zou, HF; Xiao, XZ; Guo, Z; Kong, L; Mao, XQ
关键词Monolithic Rods Immobilized Metal Affinity Chromatography Protein Iminodiacetic Acid
刊名JOURNAL OF CHROMATOGRAPHY A
2001-08-17
926期:2页:255-264
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]ION AFFINITY-CHROMATOGRAPHY ; CATION-EXCHANGE CHROMATOGRAPHY ; REVERSED-PHASE CHROMATOGRAPHY ; RIGID POLYMER MONOLITHS ; CAPILLARY ELECTROCHROMATOGRAPHY ; STATIONARY-PHASE ; POROUS PROPERTIES ; ESCHERICHIA-COLI ; CONTINUOUS BEDS ; MEMBRANE
英文摘要Continuous rod of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) was prepared by a free radical polymerization within the confines of a stainless-steel column. The epoxide groups of the rod were modified by a reaction with iminodiacetic acid (IDA) that affords the active site to form metal IDA chelates used for immobilized metal affinity chromatography (IMAC). The efficiency of coupling of IDA to the epoxide-contained matrix was studied as a function of reaction time and temperature. High-performance separation of proteins, based on immobilized different metals on the column, were described. The influence of pH on the adsorption capacity of bovine serum albumin on the CU2+-IDA continuous rod column was investigated in the range from 5.0 to 9.0. Purification of lysozyme from egg white and human serum albumin (HSA) on the commercially available HSA solution were performed on the naked IDA and Cu2+-IDA continuous rod columns, respectively; and the purity of the obtained fractions was detected by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry. (C) 2001 Elsevier Science B.V. All rights reserved.
语种英语
WOS记录号WOS:000170684200003
引用统计
被引频次:104[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/138900
专题中国科学院大连化学物理研究所
作者单位Acad Sinica, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
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Luo, QZ,Zou, HF,Xiao, XZ,et al. Chromatographic separation of proteins on metal immobilized iminodiacetic acid-bound molded monolithic rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate)[J]. JOURNAL OF CHROMATOGRAPHY A,2001,926(2):255-264.
APA Luo, QZ,Zou, HF,Xiao, XZ,Guo, Z,Kong, L,&Mao, XQ.(2001).Chromatographic separation of proteins on metal immobilized iminodiacetic acid-bound molded monolithic rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate).JOURNAL OF CHROMATOGRAPHY A,926(2),255-264.
MLA Luo, QZ,et al."Chromatographic separation of proteins on metal immobilized iminodiacetic acid-bound molded monolithic rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate)".JOURNAL OF CHROMATOGRAPHY A 926.2(2001):255-264.
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