中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Homogeneous time-resolved fluorescence DNA hybridization assay by DNA-mediated formation of an EDTA-Eu(III)-beta-diketonate ternary complex
作者: Wang, GL;  Yuan, JL;  Matsumoto, K;  Hu, Z
刊名: ANALYTICAL BIOCHEMISTRY
发表日期: 2001-12-15
DOI: 10.1006/abio.2001.5354
卷: 299, 期:2, 页:169-172
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A sensitive homogenous time-resolved fluorescence DNA hybridization assay method based on the formation of an EDTA-Eu3+-beta-diketonate ternary complex in the DNA hybrid was developed. The new approach combined the use of two DNA probes whose sequences compose the whole complementary strand to the target DNA, in which one probe was labeled with an EDTA-Eu3+ complex on the 5'-terminus and the other, labeled with a bidentate beta-diketone on the 3'-terminus. After hybridization of two DNA probes with target DNA, EDTA-Eu3+ and beta-diketone come close to each other, and an EDTA-Eu3+-beta-diketonate ternary complex with a strong and long-lived fluorescence was formed; thus the target DNA was detected sensitively with a detection limit of 6 pM (0.6 fmol per assay) by time-resolved fluorescence measurement. In the absence of the target DNA, due to the poor stability of bidentate beta-diketonate-Eu3+ complex in very diluted solution, only a small amount of ternary fluorescence complex was formed. (C) 2001 Elsevier Science.
关键词[WOS]: ENERGY-TRANSFER ;  BIOTECHNOLOGY ;  PROBES ;  GENE
语种: 英语
WOS记录号: WOS:000173223300007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138909
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Lanzhou Univ, Dept Chem, Lanzhou 730001, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116012, Peoples R China
3.Waseda Univ, Dept Chem, Shinjuku Ku, Tokyo 1698555, Japan

Recommended Citation:
Wang, GL,Yuan, JL,Matsumoto, K,et al. Homogeneous time-resolved fluorescence DNA hybridization assay by DNA-mediated formation of an EDTA-Eu(III)-beta-diketonate ternary complex[J]. ANALYTICAL BIOCHEMISTRY,2001,299(2):169-172.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Wang, GL]'s Articles
 [Yuan, JL]'s Articles
 [Matsumoto, K]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Wang, GL]‘s Articles
 [Yuan, JL]‘s Articles
 [Matsumoto, K]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace