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题名: Analysis of urinary nucleosides by micellar electrokinetic chromatography
作者: Zheng, YF;  Zhang, Y;  Liu, DY;  Guo, XL;  Mei, SR;  Xiong, JH;  Kong, HW;  Zhang, C;  Xu, GW
关键词: micellar electrokinetic chromatography ;  nucleosides ;  urine
刊名: CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
发表日期: 2001-06-01
卷: 22, 期:6, 页:912-915
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: A micellar electrokinetic chromatography method for identification and quantitation of nucleosides in urine has been optimized. After nucleosides were extracted from human urine on phenyl boronic acid affinity chromatography column, the analysis was performed in an uncoated 52 cm X 50 mum i. d. capillary tube using a 300 mmol/L SDS-25 mmol/L borate-50 mmol/L phosphate buffer (pH = 6. 96), 0. 5 Psi X 15 s injection, 7. 0 kV voltage and UV detection at 254 nm, By using the MEKC method developed, 14 normal and modified nucleosides in 28 normal urines were quantified to establish the reference ranges. The result shows that this MEKC method is reliable, sensitive, efficient and suitable for clinical application.
关键词[WOS]: CAPILLARY-ELECTROPHORESIS ;  CANCER-PATIENTS ;  TUMOR-MARKER ;  EXCRETION
语种: 英语
WOS记录号: WOS:000169605000010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138913
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
2.Dalian Univ Med, Dept Pathol, Dalian 116033, Peoples R China

Recommended Citation:
Zheng, YF,Zhang, Y,Liu, DY,et al. Analysis of urinary nucleosides by micellar electrokinetic chromatography[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2001,22(6):912-915.
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