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题名: Preparation and characterization of protein A immobilized on molded monolithic rod of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) for affinity chromatography
作者: Luo, QZ;  Zou, HF;  Wang, HL;  Mao, XQ;  Kong, L;  Ni, JY
关键词: affinity chromatography ;  polymer ;  monolithic ;  protein A ;  human immunoglobulin G
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2001-05-01
卷: 29, 期:5, 页:497-501
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Reactive continuous rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) have been prepared by "in-situ" copolymerization of the monomers in the presence of porogenic dilute, and protein A was immobilized on the continuous rods directly or through a 11 carbon atom space arm with molded synthetic methods. The properties of these two rods columns were characterized and the results showed that the medium with space arm had some extent hydrophobicity. The affinity column without space arm was used to determine the human immunoglobulin G (HIgG) in human serum. The correlative coefficient of the calibration curve was 0.999; The effect of the flow rate on the pressure of the continuous rods was also investigated. The subclasses of HIgG were separated by the use of a pH gradient.
关键词[WOS]: SEPARATION ;  MEMBRANE
语种: 英语
WOS记录号: WOS:000168992300001
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138929
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Acad Sinica, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Luo, QZ,Zou, HF,Wang, HL,et al. Preparation and characterization of protein A immobilized on molded monolithic rod of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) for affinity chromatography[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2001,29(5):497-501.
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