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题名: Separation of deoxyribonucleic acid in glucose-hydroxy-propylmethyl cellulose solution by capillary electrophoresis
作者: Jin, Y;  Lin, BC;  Fung, YS
关键词: capillary electrophoresis ;  deoxyribonucleic acid fragment ;  glucose
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2001-05-01
卷: 29, 期:5, 页:502-506
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Glycerol, mannitol has been used as additives for the separation of deoxyribonucleic acid (DNA) fragments in entangled solution by capillary electrophoresis. Differing from glycerol and mannitol, the structure of glucose is a ring with 5 hydroxyl groups. This structure helps it easily to form complex with boric acid and hydroxypropyl methylcellulose (HPMC). On the other hand, glucose has large space obstacle compared with glycerol and mannitol. So glucose can markedly improve separation of DNA in HPMC solution by capillary electrophoresis compared with other additives. The factors such as boric acid concentration and pH, influencing formation of complex of HPMC and glucose were studied. High concentration of boric acid could improve the DNA separation in glucose-HPMC matrix system. The optimization pH to separate DNA in HPMC-glucose solution by capillary electrophoresis was 8.3.
关键词[WOS]: POLYMER-SOLUTION ;  DNA FRAGMENTS
语种: 英语
WOS记录号: WOS:000168992300002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138942
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China

Recommended Citation:
Jin, Y,Lin, BC,Fung, YS. Separation of deoxyribonucleic acid in glucose-hydroxy-propylmethyl cellulose solution by capillary electrophoresis[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2001,29(5):502-506.
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