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Separation of deoxyribonucleic acid in glucose-hydroxy-propylmethyl cellulose solution by capillary electrophoresis
Jin, Y; Lin, BC; Fung, YS
关键词Capillary Electrophoresis Deoxyribonucleic Acid Fragment Glucose
刊名CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2001-05-01
29期:5页:502-506
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]POLYMER-SOLUTION ; DNA FRAGMENTS
英文摘要Glycerol, mannitol has been used as additives for the separation of deoxyribonucleic acid (DNA) fragments in entangled solution by capillary electrophoresis. Differing from glycerol and mannitol, the structure of glucose is a ring with 5 hydroxyl groups. This structure helps it easily to form complex with boric acid and hydroxypropyl methylcellulose (HPMC). On the other hand, glucose has large space obstacle compared with glycerol and mannitol. So glucose can markedly improve separation of DNA in HPMC solution by capillary electrophoresis compared with other additives. The factors such as boric acid concentration and pH, influencing formation of complex of HPMC and glucose were studied. High concentration of boric acid could improve the DNA separation in glucose-HPMC matrix system. The optimization pH to separate DNA in HPMC-glucose solution by capillary electrophoresis was 8.3.
语种英语
WOS记录号WOS:000168992300002
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/138942
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China
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GB/T 7714
Jin, Y,Lin, BC,Fung, YS. Separation of deoxyribonucleic acid in glucose-hydroxy-propylmethyl cellulose solution by capillary electrophoresis[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2001,29(5):502-506.
APA Jin, Y,Lin, BC,&Fung, YS.(2001).Separation of deoxyribonucleic acid in glucose-hydroxy-propylmethyl cellulose solution by capillary electrophoresis.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,29(5),502-506.
MLA Jin, Y,et al."Separation of deoxyribonucleic acid in glucose-hydroxy-propylmethyl cellulose solution by capillary electrophoresis".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 29.5(2001):502-506.
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