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题名: Immunoassay by graphite furnace atomic absorption spectrometry using a metal chelate as a label
作者: Wang, GL;  Yuan, JL;  Gong, BL;  Matsumoto, K;  Hu, ZD
关键词: immunoassay ;  atomic absorption spectrometry ;  alpha-fetoprotein ;  bensulfuron-methyl
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2001-12-03
卷: 448, 期:1-2, 页:165-172
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A new immunoassay method was developed by using graphite furnace atomic absorption spectrometry and EDTA-Cd2+ chelate as a label. The new approach combined the use of EDTA-Cd2+ chelate labeled streptavidin (SA), biotinylated antibody or antigen, and graphite furnace atomic absorption detection for both sandwich-type immunoassay and competitive-type immunoassay. The measurements of alpha -fetoprotein (AFP) in human sera and bensulfuron-methyl (BSM) in water were used as the models to evaluate the usefulness of the method. The assays were carried out with 96-well microtiter plate as solid-phase carrier. After the immune reaction and biotin-streptavidin binding reaction, a solution of 0.2 M HNO3 was added to each well to dissociate Cd2+ in the immune complex on the solid-phase into the solution. The antigen concentration, was thus, determined by measuring the Cd2+ absorbance of the solution with graphite furnace atomic absorption spectrometry. The present method gives detection limits of 0.12 ng ml(-1) for AFP and 0.95 ng ml(-1) for BSM, respectively. The coefficient variations (CVs) of the method are less than 8.0%, and the recoveries are in the range of 90-110%. The concentrations of AFP in 23 human serum samples were determined, and the results were compared with those of the independently determined by time-resolved fluoroimmunoassay method. A good correlation was obtained with a correlation coefficient of 0.993. (C) 2001 Elsevier Science B.V. All rights reserved.
关键词[WOS]: TIME-RESOLVED FLUORESCENCE ;  ALPHA-FETOPROTEIN ;  BIOTECHNOLOGY
语种: 英语
WOS记录号: WOS:000172458400018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138943
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116012, Peoples R China
2.Lanzhou Univ, Dept Chem, Lanzhou 730001, Peoples R China
3.Waseda Univ, Dept Chem, Shinjuku Ku, Tokyo 1698555, Japan

Recommended Citation:
Wang, GL,Yuan, JL,Gong, BL,et al. Immunoassay by graphite furnace atomic absorption spectrometry using a metal chelate as a label[J]. ANALYTICA CHIMICA ACTA,2001,448(1-2):165-172.
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