中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: An improved derivatization method for sensitive determination of fatty acids by high-performance liquid chromatography using 9-(2-hydroxylethyl)-carbazole as derivatization reagent with fluorescence detection
作者: You, JM;  Zhang, WB;  Jia, XL;  Zhang, YK
关键词: column liquid chromatography ;  fluorescent detection ;  9-(2-hydroxyethyl)-carbazole ;  fatty acids ;  plasma samples
刊名: CHROMATOGRAPHIA
发表日期: 2001-09-01
卷: 54, 期:5-6, 页:316-322
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Tagging techniques with reagents used for fluorescent detection for short and long-chain fatty acids using high-performance liquid chromatography are evaluated in terms of the fagging reactions, handing, flexibility, stability of the reagents. Emphasis is given to the applications of the tagging techniques to relatively high molecular mass fatty acids. The fatty acids or carboxylic compounds were derivatized to their corresponding esters with 9-(2-hydroxy ethyl)-carbazole (HEC) in acetronitrile at 60 degreesC with N, N'-carbonyldiimidazole (CDI) as a coupling agent in the presence of 4-dimethylaminopyridine (DMAP). A mixture of esters of C-1-C-20 fatty acids was completely separated within 45 min using gradient elution on a reversed-phase C-18 column. The maximum fluorescence emission for the derivatized fatty acids is at 365 nm (lambda (ex) 293 nm). Studies on derivatization conditions indicated that fatty acids react rapidly and smoothly with HEC in the presence of CDI and DMAP in acetonitrile to give the corresponding sensitively fluorescent derivatives. The application of this method to the analysis of long chain fatty acids in plasma is also investigated. The LC separation shows good selectivity and reproducibility for fatty acids derivatives. The relative standard deviations (n = 6) for each fatty acid derivative are < 5.0%. The defection limits are at 38 - 57 fmol levels for C-14-C-20 fatty acids and lower levels for < C-14 fatty acids.
关键词[WOS]: CARBOXYLIC-ACIDS ;  LABELING REAGENT ;  AMINO-ACIDS ;  TRIFLUOROMETHANESULFONATE ;  DERIVATIVES ;  9-ANTHRYLDIAZOMETHANE ;  PROSTAGLANDINS ;  CHLORIDE ;  SERUM
语种: 英语
WOS记录号: WOS:000171316600004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138965
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
2.Qufu Normal Univ, Dept Chem, Shandong 273165, Peoples R China

Recommended Citation:
You, JM,Zhang, WB,Jia, XL,et al. An improved derivatization method for sensitive determination of fatty acids by high-performance liquid chromatography using 9-(2-hydroxylethyl)-carbazole as derivatization reagent with fluorescence detection[J]. CHROMATOGRAPHIA,2001,54(5-6):316-322.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [You, JM]'s Articles
 [Zhang, WB]'s Articles
 [Jia, XL]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [You, JM]‘s Articles
 [Zhang, WB]‘s Articles
 [Jia, XL]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace