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Kinetics of inactivation of calf intestine alkaline phosphatase by EDTA with absorption spectrum method
Wang, JY; Peng, XJ; Yang, D; An, LJ; Hu, JH; Zheng, XF
关键词Absorption Spectrum Method Alkaline Phosphatase Kinetics Inactivation
刊名SPECTROSCOPY AND SPECTRAL ANALYSIS
2001-10-01
21期:5页:701-703
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Technology
类目[WOS]Spectroscopy
研究领域[WOS]Spectroscopy
关键词[WOS]AMINOACYLASE ; 1,10-PHENANTHROLINE ; IONS
英文摘要Calf intestinal alkaline phosphatase (EC. 3.1.3.1) is a dimeric metalloenzyme composed of two identical subunits, the each active site of which contains a tight cluster of two zinc ions and one magnesium ion. The kinetic theory of the substrate reaction during irreversible inhibition of enzyme activity previously described by Tsou has been applied for a study on the kinetics of the course of inactivation of the enzyme by EDTA. The kinetics of the substrate reaction with different concentrations of the substrate p-nitrophenylphosphate (PNPP) and inactivator EDTA suggested a competitive complexing mechanism for inactivation by EDTA, and the process of inactivation composed of the rapid initial formation of an enzyme-EDTA complex, in which the conformation of enzyme has been changed, and then zinc ions are finally removed from the enzyme.
语种英语
WOS记录号WOS:000171963200038
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/138980
专题中国科学院大连化学物理研究所
作者单位1.Dalian Univ Technol, Dept Bioengn, Dalian 116012, Peoples R China
2.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
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GB/T 7714
Wang, JY,Peng, XJ,Yang, D,et al. Kinetics of inactivation of calf intestine alkaline phosphatase by EDTA with absorption spectrum method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2001,21(5):701-703.
APA Wang, JY,Peng, XJ,Yang, D,An, LJ,Hu, JH,&Zheng, XF.(2001).Kinetics of inactivation of calf intestine alkaline phosphatase by EDTA with absorption spectrum method.SPECTROSCOPY AND SPECTRAL ANALYSIS,21(5),701-703.
MLA Wang, JY,et al."Kinetics of inactivation of calf intestine alkaline phosphatase by EDTA with absorption spectrum method".SPECTROSCOPY AND SPECTRAL ANALYSIS 21.5(2001):701-703.
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