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Kinetics of inactivation of calf intestine alkaline phosphatase by EDTA with absorption spectrum method
Wang, JY; Peng, XJ; Yang, D; An, LJ; Hu, JH; Zheng, XF
KeywordAbsorption Spectrum Method Alkaline Phosphatase Kinetics Inactivation
Source PublicationSPECTROSCOPY AND SPECTRAL ANALYSIS
2001-10-01
Volume21Issue:5Pages:701-703
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
WOS SubjectSpectroscopy
WOS Research AreaSpectroscopy
WOS KeywordAMINOACYLASE ; 1,10-PHENANTHROLINE ; IONS
AbstractCalf intestinal alkaline phosphatase (EC. 3.1.3.1) is a dimeric metalloenzyme composed of two identical subunits, the each active site of which contains a tight cluster of two zinc ions and one magnesium ion. The kinetic theory of the substrate reaction during irreversible inhibition of enzyme activity previously described by Tsou has been applied for a study on the kinetics of the course of inactivation of the enzyme by EDTA. The kinetics of the substrate reaction with different concentrations of the substrate p-nitrophenylphosphate (PNPP) and inactivator EDTA suggested a competitive complexing mechanism for inactivation by EDTA, and the process of inactivation composed of the rapid initial formation of an enzyme-EDTA complex, in which the conformation of enzyme has been changed, and then zinc ions are finally removed from the enzyme.
Language英语
WOS IDWOS:000171963200038
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/138980
Collection中国科学院大连化学物理研究所
Affiliation1.Dalian Univ Technol, Dept Bioengn, Dalian 116012, Peoples R China
2.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Wang, JY,Peng, XJ,Yang, D,et al. Kinetics of inactivation of calf intestine alkaline phosphatase by EDTA with absorption spectrum method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2001,21(5):701-703.
APA Wang, JY,Peng, XJ,Yang, D,An, LJ,Hu, JH,&Zheng, XF.(2001).Kinetics of inactivation of calf intestine alkaline phosphatase by EDTA with absorption spectrum method.SPECTROSCOPY AND SPECTRAL ANALYSIS,21(5),701-703.
MLA Wang, JY,et al."Kinetics of inactivation of calf intestine alkaline phosphatase by EDTA with absorption spectrum method".SPECTROSCOPY AND SPECTRAL ANALYSIS 21.5(2001):701-703.
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