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题名: Kinetics of inactivation of calf intestine alkaline phosphatase by EDTA with absorption spectrum method
作者: Wang, JY;  Peng, XJ;  Yang, D;  An, LJ;  Hu, JH;  Zheng, XF
关键词: absorption spectrum method ;  alkaline phosphatase ;  kinetics ;  inactivation
刊名: SPECTROSCOPY AND SPECTRAL ANALYSIS
发表日期: 2001-10-01
卷: 21, 期:5, 页:701-703
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Spectroscopy
研究领域[WOS]: Spectroscopy
英文摘要: Calf intestinal alkaline phosphatase (EC. 3.1.3.1) is a dimeric metalloenzyme composed of two identical subunits, the each active site of which contains a tight cluster of two zinc ions and one magnesium ion. The kinetic theory of the substrate reaction during irreversible inhibition of enzyme activity previously described by Tsou has been applied for a study on the kinetics of the course of inactivation of the enzyme by EDTA. The kinetics of the substrate reaction with different concentrations of the substrate p-nitrophenylphosphate (PNPP) and inactivator EDTA suggested a competitive complexing mechanism for inactivation by EDTA, and the process of inactivation composed of the rapid initial formation of an enzyme-EDTA complex, in which the conformation of enzyme has been changed, and then zinc ions are finally removed from the enzyme.
关键词[WOS]: AMINOACYLASE ;  1,10-PHENANTHROLINE ;  IONS
语种: 英语
WOS记录号: WOS:000171963200038
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138980
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Univ Technol, Dept Bioengn, Dalian 116012, Peoples R China
2.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
Wang, JY,Peng, XJ,Yang, D,et al. Kinetics of inactivation of calf intestine alkaline phosphatase by EDTA with absorption spectrum method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2001,21(5):701-703.
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