The authors used hydrophillic interaction capillary electrochromatography (CEC) to separate polar compounds. Their system had a capillary column, packed with a hydrophillic, strong cation-exchange material of poly(2-sulfoethyl aspartamide)-silica and a hydrophobic mobile phase, which typically contained 80% acetonitrile. The elution order of polar compounds in hydrophilic interaction CEC is similar to that obtained by normal-phase chromatography, and the retention of solutes increases with their hydrophillicity. The authors obtained column efficiencies of 79,000-111,000 plates/m with separation of some model polar compounds under optimal conditions. The repeatability of their system was good with relative standard deviations of approximately 0.4% and less than 0.7% for void time and solute retention factors, respectively, in 10 consecutive runs.
1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
Recommended Citation:
Ye, ML,Zou, HF,Kong, L,et al. Hydrophilic interaction capillary electrochromatography for the separation of polar compounds[J]. LC GC NORTH AMERICA,2002,19(10):41-46.
