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题名: Separation of basic, acidic and neutral compounds by capillary electrochromatography using uncharged monolithic capillary columns modified with anionic and cationic surfactants
作者: Wu, RN;  Zou, HF;  Ye, ML;  Lei, ZD;  Ni, JY
关键词: electrochromatography ;  dynamically modified stationary phase surfactant ;  uncharged monolithic column
刊名: ELECTROPHORESIS
发表日期: 2001-02-01
卷: 22, 期:3, 页:544-551
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A mode of capillary electrochromatography (CEC), based on the dynamical adsorption of surfactants on the uncharged monolithic stationary phases has been developed. The monolithic stationary phase, obtained by the in situ polymerization of butyl methacrylate with ethylene dimethacrylate, was dynamically modified with an ionic surfactant such as the long-chain quaternary ammonium salt of cetyltrimethylammonium bromide (CTAB) and long-chain sodium sulfate of sodium dodecyl sulfate (SDS). The ionic surfactant was adsorbed on the surface of polymeric monolith by hydrophobic interaction, and the ionic groups used to generate the electroosmotic flow (EOF). The electroosmotic mobility through these capillary columns increased with increasing the content of ionic surfactants in the mobile phase. In this way, the synthesis of the monolithic stationary phase with binary monomers can be controlled more easily than that with ternary monomers, one of which should be an ionic monomer to generate EOF. Furthermore, it is more convenient to change the direction and magnitude of EOF by changing the concentration of cationic or anionic surfactants in this system. An efficiency of monolithic capillary columns with more than 140 000 plates per meter for neutral compounds has been obtained, and the relative standard deviations observed for t(0) and retention factors of neutral solutes were about 0.22% and less than 0.56% for ten consecutive runs, respectively. Effects of mobile phase composition on the EOF of the column and the retention values of the neutral solutes were investigated. Simultaneous separation of basic, neutral and acidic compounds has been achieved.
关键词[WOS]: RIGID POLYMER MONOLITHS ;  PERFORMANCE LIQUID-CHROMATOGRAPHY ;  DYNAMICALLY MODIFIED SILICA ;  ELECTROOSMOTIC FLOW ;  ZONE ELECTROPHORESIS ;  STATIONARY PHASES ;  ION-EXCHANGE ;  SELECTIVITY ;  MEDIA ;  BASES
语种: 英语
WOS记录号: WOS:000167362400026
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138995
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Wu, RN,Zou, HF,Ye, ML,et al. Separation of basic, acidic and neutral compounds by capillary electrochromatography using uncharged monolithic capillary columns modified with anionic and cationic surfactants[J]. ELECTROPHORESIS,2001,22(3):544-551.
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