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Study on the direct interaction between alkaline phophatase and Cu(II) ions by spectral analysis
Wang, JY; Zheng, XF; An, LJ; Gai, HW; Hu, JH
KeywordEpr Spectroscopy Alkaline Phosphatase Cu(Ii) Ions Interaction
Source PublicationSPECTROSCOPY AND SPECTRAL ANALYSIS
2001-08-01
Volume21Issue:4Pages:432-434
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
WOS SubjectSpectroscopy
WOS Research AreaSpectroscopy
WOS KeywordPHOSPHATASE
AbstractIn this paper, the results of EPR, VIS and enzyme activity show that alkaline phophatase from calf intest directly interacted with additional Cu( II ). The added Cu( II ) enter into A site of active sites of AKP which decrease enzyme activity. With increase of pH value, the decrease in the intensity of the Cu( II ) EPR signal and the change of place signal in VIS were observed. These results suggest that Cu( II ) migrate in the active center of the enzyme.
Language英语
WOS IDWOS:000170980700005
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/139037
Collection中国科学院大连化学物理研究所
Affiliation1.Dalian Univ Technol, Dept Bioengn, Dalian 116012, Peoples R China
2.Dalian Univ Technol, Inst Bioengn, Dalian 116622, Peoples R China
3.Acad Sinica, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Wang, JY,Zheng, XF,An, LJ,et al. Study on the direct interaction between alkaline phophatase and Cu(II) ions by spectral analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2001,21(4):432-434.
APA Wang, JY,Zheng, XF,An, LJ,Gai, HW,&Hu, JH.(2001).Study on the direct interaction between alkaline phophatase and Cu(II) ions by spectral analysis.SPECTROSCOPY AND SPECTRAL ANALYSIS,21(4),432-434.
MLA Wang, JY,et al."Study on the direct interaction between alkaline phophatase and Cu(II) ions by spectral analysis".SPECTROSCOPY AND SPECTRAL ANALYSIS 21.4(2001):432-434.
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