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Studies on rapid micro-scale peptide mapping analysis using a capillary micro-reactor
Guo, Z; Zhang, QC; Lei, ZD; Kong, L; Mao, XQ; Zou, HF
关键词Nano-liter Micro-reactor Capillary Immobilized Enzyme Maldi-tof-ms Peptide Mapping Analysis
刊名CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
2002-07-01
23期:7页:1277-1280
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]ELECTROCHROMATOGRAPHY
英文摘要Peptide mapping analysis by combining the enzymatic digestion and the MALDI-TOF-MS has emerged as a powerful tool for identification and characterization of protein. We describe a nanoliter microreactor prepared with immobilization of enzyme on the porous wall of a capillary. The advantages of the prepared micro-reactor for digestion of the proteins are the elimination of enzyme autolysis and concentration of the micro-sample. Horse heart cytochrome c is selected as the target protein for the evaluation of micro-reactor, and it is found that about 0.1 picomole of protein analyte could routinely be digested for peptide mapping analysis and even femtomole of protein can be well characterized.
语种英语
WOS记录号WOS:000176908400015
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被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/139061
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&S Ctr, Dalian 116011, Peoples R China
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GB/T 7714
Guo, Z,Zhang, QC,Lei, ZD,et al. Studies on rapid micro-scale peptide mapping analysis using a capillary micro-reactor[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2002,23(7):1277-1280.
APA Guo, Z,Zhang, QC,Lei, ZD,Kong, L,Mao, XQ,&Zou, HF.(2002).Studies on rapid micro-scale peptide mapping analysis using a capillary micro-reactor.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,23(7),1277-1280.
MLA Guo, Z,et al."Studies on rapid micro-scale peptide mapping analysis using a capillary micro-reactor".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 23.7(2002):1277-1280.
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