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题名: Studies on rapid micro-scale peptide mapping analysis using a capillary micro-reactor
作者: Guo, Z;  Zhang, QC;  Lei, ZD;  Kong, L;  Mao, XQ;  Zou, HF
关键词: nano-liter micro-reactor ;  capillary immobilized enzyme ;  MALDI-TOF-MS ;  peptide mapping analysis
刊名: CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
发表日期: 2002-07-01
卷: 23, 期:7, 页:1277-1280
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: Peptide mapping analysis by combining the enzymatic digestion and the MALDI-TOF-MS has emerged as a powerful tool for identification and characterization of protein. We describe a nanoliter microreactor prepared with immobilization of enzyme on the porous wall of a capillary. The advantages of the prepared micro-reactor for digestion of the proteins are the elimination of enzyme autolysis and concentration of the micro-sample. Horse heart cytochrome c is selected as the target protein for the evaluation of micro-reactor, and it is found that about 0.1 picomole of protein analyte could routinely be digested for peptide mapping analysis and even femtomole of protein can be well characterized.
关键词[WOS]: ELECTROCHROMATOGRAPHY
语种: 英语
WOS记录号: WOS:000176908400015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139061
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&S Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Guo, Z,Zhang, QC,Lei, ZD,et al. Studies on rapid micro-scale peptide mapping analysis using a capillary micro-reactor[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2002,23(7):1277-1280.
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