DICP OpenIR
Studies on rapid micro-scale peptide mapping analysis using a capillary micro-reactor
Guo, Z; Zhang, QC; Lei, ZD; Kong, L; Mao, XQ; Zou, HF
KeywordNano-liter Micro-reactor Capillary Immobilized Enzyme Maldi-tof-ms Peptide Mapping Analysis
Source PublicationCHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
2002-07-01
Volume23Issue:7Pages:1277-1280
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordELECTROCHROMATOGRAPHY
AbstractPeptide mapping analysis by combining the enzymatic digestion and the MALDI-TOF-MS has emerged as a powerful tool for identification and characterization of protein. We describe a nanoliter microreactor prepared with immobilization of enzyme on the porous wall of a capillary. The advantages of the prepared micro-reactor for digestion of the proteins are the elimination of enzyme autolysis and concentration of the micro-sample. Horse heart cytochrome c is selected as the target protein for the evaluation of micro-reactor, and it is found that about 0.1 picomole of protein analyte could routinely be digested for peptide mapping analysis and even femtomole of protein can be well characterized.
Language英语
WOS IDWOS:000176908400015
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/139061
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&S Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Guo, Z,Zhang, QC,Lei, ZD,et al. Studies on rapid micro-scale peptide mapping analysis using a capillary micro-reactor[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2002,23(7):1277-1280.
APA Guo, Z,Zhang, QC,Lei, ZD,Kong, L,Mao, XQ,&Zou, HF.(2002).Studies on rapid micro-scale peptide mapping analysis using a capillary micro-reactor.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,23(7),1277-1280.
MLA Guo, Z,et al."Studies on rapid micro-scale peptide mapping analysis using a capillary micro-reactor".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 23.7(2002):1277-1280.
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