Synthesis of a silica-bonded bovine serum albumin carbonyl chiral stationary phase for high-performance liquid chromatographic resolution of enantiomers
An HPLC chiral stationary phase was synthesized by the in situ covalent immobilization of bovine serum albumin on a aminopropyl silica packed column activated with 1,1-carbonyldiimidazole in a short time. The effects of mobile phase pH and the content of 1-propanol on k' and alpha of D,L-tryptophan were investigated, and the resolution of seven chiral compounds was successfully achieved.
1.Xinjiang Univ, Dept Chem, Urumqi 830024, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
Recommended Citation:
Feng, S,Zhang, Q,Zou, HF,et al. Synthesis of a silica-bonded bovine serum albumin carbonyl chiral stationary phase for high-performance liquid chromatographic resolution of enantiomers[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2002,23(7):1251-1254.
