DICP OpenIR
Quantitative measurement of 17 beta-estradiol and estriol in river water by time-resolved fluoroimmunoassay
Majima, K; Fukui, T; Yuan, JL; Wang, GL; Matsumoto, K
刊名ANALYTICAL SCIENCES
2002-08-01
18期:8页:869-874
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]DIKETONATE-EUROPIUM CHELATE ; CHROMATOGRAPHY-MASS-SPECTROMETRY ; TREATED SEWAGE EFFLUENT ; SOLID-PHASE EXTRACTION ; ESTROGENIC CHEMICALS ; AQUATIC ENVIRONMENT ; STW EFFLUENT ; LABEL ; IDENTIFICATION ; ESTRADIOL
英文摘要A sensitive method for detecting 17beta-estradiol (E2) and estriol (E3) in river water has been developed, based on the time-resolved fluoroimmunoassay by using a fluorescent europium chelate label, 4,4'-bis(1",1",1",2",2",3",3"-heptafluoro-4",6"-hexanedion-6"-yl)-chlorosulfo-o-terphenyl (BHHCT)-Eu3+. In the E2 assay, microtiter plates were coated with the E2-bovine serum albumin (BSA) conjugate. The anti-17beta-estradiol antibody, the biotinylated goat anti-rabbit IgG antibody and the BHHCT-Eu3+ labeled streptavidin (SA)-BSA conjugate were used. In the E3 assay, the goat anti-rabbit IgG antibody was coated on a microtiter plate. The anti-estriol antibody and the BHHCT-Eu3+ labeled E3-BSA conjugate were used. The detection limits for E2 and E3 were 2.3 pg/ml and 4.3 pg/ml, respectively, and the analytical recoveries were 95-120%. Quantitative measurement of estrogens in river water was carried out for Kanda River (Tokyo, Japan) by using the method. The E2 and E3 levels were 32 pg/ml and 5.5 pg/ml, respectively. The detection limits of the present method are in the same orders of magnitude as those of ELISA for E2, and are 1-2 orders of magnitude better for E3.
语种英语
WOS记录号WOS:000177500200002
引用统计
被引频次:27[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/139086
专题中国科学院大连化学物理研究所
作者单位1.Waseda Univ, Adv Res Inst Sci & Engn, Dept Chem, Shinju Ku, Tokyo 1698555, Japan
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116012, Peoples R China
推荐引用方式
GB/T 7714
Majima, K,Fukui, T,Yuan, JL,et al. Quantitative measurement of 17 beta-estradiol and estriol in river water by time-resolved fluoroimmunoassay[J]. ANALYTICAL SCIENCES,2002,18(8):869-874.
APA Majima, K,Fukui, T,Yuan, JL,Wang, GL,&Matsumoto, K.(2002).Quantitative measurement of 17 beta-estradiol and estriol in river water by time-resolved fluoroimmunoassay.ANALYTICAL SCIENCES,18(8),869-874.
MLA Majima, K,et al."Quantitative measurement of 17 beta-estradiol and estriol in river water by time-resolved fluoroimmunoassay".ANALYTICAL SCIENCES 18.8(2002):869-874.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Majima, K]的文章
[Fukui, T]的文章
[Yuan, JL]的文章
百度学术
百度学术中相似的文章
[Majima, K]的文章
[Fukui, T]的文章
[Yuan, JL]的文章
必应学术
必应学术中相似的文章
[Majima, K]的文章
[Fukui, T]的文章
[Yuan, JL]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。