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题名: Quantitative measurement of 17 beta-estradiol and estriol in river water by time-resolved fluoroimmunoassay
作者: Majima, K;  Fukui, T;  Yuan, JL;  Wang, GL;  Matsumoto, K
刊名: ANALYTICAL SCIENCES
发表日期: 2002-08-01
卷: 18, 期:8, 页:869-874
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A sensitive method for detecting 17beta-estradiol (E2) and estriol (E3) in river water has been developed, based on the time-resolved fluoroimmunoassay by using a fluorescent europium chelate label, 4,4'-bis(1",1",1",2",2",3",3"-heptafluoro-4",6"-hexanedion-6"-yl)-chlorosulfo-o-terphenyl (BHHCT)-Eu3+. In the E2 assay, microtiter plates were coated with the E2-bovine serum albumin (BSA) conjugate. The anti-17beta-estradiol antibody, the biotinylated goat anti-rabbit IgG antibody and the BHHCT-Eu3+ labeled streptavidin (SA)-BSA conjugate were used. In the E3 assay, the goat anti-rabbit IgG antibody was coated on a microtiter plate. The anti-estriol antibody and the BHHCT-Eu3+ labeled E3-BSA conjugate were used. The detection limits for E2 and E3 were 2.3 pg/ml and 4.3 pg/ml, respectively, and the analytical recoveries were 95-120%. Quantitative measurement of estrogens in river water was carried out for Kanda River (Tokyo, Japan) by using the method. The E2 and E3 levels were 32 pg/ml and 5.5 pg/ml, respectively. The detection limits of the present method are in the same orders of magnitude as those of ELISA for E2, and are 1-2 orders of magnitude better for E3.
关键词[WOS]: DIKETONATE-EUROPIUM CHELATE ;  CHROMATOGRAPHY-MASS-SPECTROMETRY ;  TREATED SEWAGE EFFLUENT ;  SOLID-PHASE EXTRACTION ;  ESTROGENIC CHEMICALS ;  AQUATIC ENVIRONMENT ;  STW EFFLUENT ;  LABEL ;  IDENTIFICATION ;  ESTRADIOL
语种: 英语
WOS记录号: WOS:000177500200002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139086
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Waseda Univ, Adv Res Inst Sci & Engn, Dept Chem, Shinju Ku, Tokyo 1698555, Japan
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116012, Peoples R China

Recommended Citation:
Majima, K,Fukui, T,Yuan, JL,et al. Quantitative measurement of 17 beta-estradiol and estriol in river water by time-resolved fluoroimmunoassay[J]. ANALYTICAL SCIENCES,2002,18(8):869-874.
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