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题名: Preparation and characterization of protein A immobilized on monolithic capillary column for affinity chromatography
作者: Pan, ZF;  Mo, WM;  Wu, RA;  Luo, QZ;  Ye, ML;  Zou, HF
关键词: affinity chromatography ;  monolithic capillary column ;  protein A ;  human immunoglobulin G
刊名: CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
发表日期: 2002-11-01
卷: 23, 期:11, 页:2070-2072
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: An assay technique for the determination of the human IgG in human plasma has been developed by utilizing. protein A immobilized monolithic capillary column. The affinity chromatography experiment was performed on a capillary electrophoresis instrument by using its pressure system as the driving force. Reactive monolithic capillary columns have been prepared, by in-situ copolymerization of the monomers in the presence of porogenic dilute, and protein A was immobilized on the monolithic rods directly or;through a 11 carbon atom space I arm with molded synthetic methods. The non-specific adsorption of two kinds of media has been studied and-the results showed that the medium without space arm gives very low non-specific adsorption of BSA. The affinity column without space arm was used to determine the human immunoglobulin G (HIgG) in human serums The correlative. coefficient of the calibration curve reaches 0.998 7. The total time of rapid analysis was less than 0.7 min. The consumption of eluent buffer and sample is much low than by conventional HPLC.
关键词[WOS]: IMMUNOAFFINITY CHROMATOGRAPHY ;  IMMUNOGLOBULIN-G ;  BIOMOLECULES ;  PURIFICATION ;  SEPARATION ;  POLYMERS ;  SUPPORTS
语种: 英语
WOS记录号: WOS:000179416200015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139096
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
2.Zhejiang Univ Technol, Coll Chem Engn, Hangzhou 310014, Peoples R China

Recommended Citation:
Pan, ZF,Mo, WM,Wu, RA,et al. Preparation and characterization of protein A immobilized on monolithic capillary column for affinity chromatography[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2002,23(11):2070-2072.
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