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题名: Preparation and chromatographic behavior of acetate-fiber filter rods with dye affinity ligands
作者: Li, JH;  Wang, JD;  Liu, XL
关键词: affinity chromatography ;  acetate fiber filter rod ;  cibacron blue F3GA and reactive red 120 ;  albumin
刊名: CHROMATOGRAPHIA
发表日期: 2002-10-01
卷: 56, 期:7-8, 页:401-406
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The dyes cibacron blue F3GA and reactive red 120 have been immobilized on acetate fiber filter rods to produce potential affinity matrixes. The isothermal adsorption of bovine serum albumin or human serum albumin on these matrixes was investigated and proved to conform to the Freundlich equation. In the static adsorption of human plasma the adsorption capacity for human serum albumin was 12.5 mg g(-1) fiber filter and one bond was observed in SDS-PAGE electrophoresis of human serum albumin isolated by the immobilized cibacron blue F3GA filter rod. Ligand utilization efficiencies and breakthrough volumes were obtained for adsorption of HSA on the cibacron blue F3GA filter rod when the feed-rates were from 0.5 to 6 mL min(-1). In the affinity chromatography of human plasma the yield of human serum albumin was 1.27 g per 100 mL plasma.
关键词[WOS]: PURIFICATION ;  PROTEINS ;  DESIGN
语种: 英语
WOS记录号: WOS:000178836600002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139121
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China

Recommended Citation:
Li, JH,Wang, JD,Liu, XL. Preparation and chromatographic behavior of acetate-fiber filter rods with dye affinity ligands[J]. CHROMATOGRAPHIA,2002,56(7-8):401-406.
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