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Screening and analysis of permeable compounds in Radix Angelica Sinensis with immobilized liposome chromatography
Mao, XQ; Kong, L; Luo, QZ; Li, X; Zou, HF
KeywordRadix Angelica Sinensis Immobilized Liposome Chromatography Permeable Compounds
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2002-11-05
Volume779Issue:2Pages:331-339
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordHUMAN SERUM-ALBUMIN ; BIOLOGICALLY-ACTIVE COMPOUNDS ; DRUG-MEMBRANE INTERACTIONS ; MOLECULAR BIOCHROMATOGRAPHY ; BIOMEMBRANE CHROMATOGRAPHY ; AFFINITY-CHROMATOGRAPHY ; STATIONARY-PHASE ; CACO-2 CELLS ; COEFFICIENTS ; ABSORPTION
AbstractImmobilized liposome chromatography (ILC) was used to screen and analyze permeable compounds in traditional Chinese medicines (TCMs), testing extracts from Radix Angelica Sinensis. More than 10 peaks were resolved based on their interactions with the ILC stationary phase, a system which mimics biomembranes; this means that more than 10 components in Radix Angelica Sinensis extract have significant retention on an ILC column. Two of them, ligustilide and ferulic acid, were identified from their MS spectrum and with standard samples. A possible molecular structure of another component retained on ILC was also preliminarily identified as 3-butylidene-4,5-dihydro-2(1,3H)-1-isobenzofuranoI according to its MS spectrum, hydrophobicity and H-1 NMR spectrum. Of all detected components, ligustilide had the best penetration ability through the biomembrane. The effects of pH, column temperature, and ionic strength on the chromatography of methanolic extracts of Radix Angelica Sinensis were also investigated. It was found that the separation selectivity on ILC is strongly affected by the eluent pH, but only slightly by the column temperature and ionic strength. (C) 2002 Elsevier Science B.V. All rights reserved.
Language英语
WOS IDWOS:000178936700018
Citation statistics
Cited Times:75[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/139165
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Mao, XQ,Kong, L,Luo, QZ,et al. Screening and analysis of permeable compounds in Radix Angelica Sinensis with immobilized liposome chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2002,779(2):331-339.
APA Mao, XQ,Kong, L,Luo, QZ,Li, X,&Zou, HF.(2002).Screening and analysis of permeable compounds in Radix Angelica Sinensis with immobilized liposome chromatography.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,779(2),331-339.
MLA Mao, XQ,et al."Screening and analysis of permeable compounds in Radix Angelica Sinensis with immobilized liposome chromatography".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 779.2(2002):331-339.
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