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题名: Screening and analysis of permeable compounds in Radix Angelica Sinensis with immobilized liposome chromatography
作者: Mao, XQ;  Kong, L;  Luo, QZ;  Li, X;  Zou, HF
关键词: Radix Angelica Sinensis ;  immobilized liposome chromatography ;  permeable compounds
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2002-11-05
卷: 779, 期:2, 页:331-339
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Immobilized liposome chromatography (ILC) was used to screen and analyze permeable compounds in traditional Chinese medicines (TCMs), testing extracts from Radix Angelica Sinensis. More than 10 peaks were resolved based on their interactions with the ILC stationary phase, a system which mimics biomembranes; this means that more than 10 components in Radix Angelica Sinensis extract have significant retention on an ILC column. Two of them, ligustilide and ferulic acid, were identified from their MS spectrum and with standard samples. A possible molecular structure of another component retained on ILC was also preliminarily identified as 3-butylidene-4,5-dihydro-2(1,3H)-1-isobenzofuranoI according to its MS spectrum, hydrophobicity and H-1 NMR spectrum. Of all detected components, ligustilide had the best penetration ability through the biomembrane. The effects of pH, column temperature, and ionic strength on the chromatography of methanolic extracts of Radix Angelica Sinensis were also investigated. It was found that the separation selectivity on ILC is strongly affected by the eluent pH, but only slightly by the column temperature and ionic strength. (C) 2002 Elsevier Science B.V. All rights reserved.
关键词[WOS]: HUMAN SERUM-ALBUMIN ;  BIOLOGICALLY-ACTIVE COMPOUNDS ;  DRUG-MEMBRANE INTERACTIONS ;  MOLECULAR BIOCHROMATOGRAPHY ;  BIOMEMBRANE CHROMATOGRAPHY ;  AFFINITY-CHROMATOGRAPHY ;  STATIONARY-PHASE ;  CACO-2 CELLS ;  COEFFICIENTS ;  ABSORPTION
语种: 英语
WOS记录号: WOS:000178936700018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139165
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Mao, XQ,Kong, L,Luo, QZ,et al. Screening and analysis of permeable compounds in Radix Angelica Sinensis with immobilized liposome chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2002,779(2):331-339.
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