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Determination of biogenic Amines by RPHPLC with fluorescent detection after derivatization with 2-(9-carbazole)ethyl chloroformate (CEOC)
You, JM; Zhang, YK
KeywordColumn Liquid Chromatography Fluorescent Detection 2-(9-carbazole)Ethyl Chloroformate Biogenic Amines
Source PublicationCHROMATOGRAPHIA
2002-07-01
Volume56Issue:1-2Pages:43-50
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordLIQUID-CHROMATOGRAPHIC DETERMINATION ; AUTOMATED PRECOLUMN DERIVATIZATION ; RP-HPLC ; O-PHTHALDIALDEHYDE ; CHLORIDE ; ACIDS ; FOODS ; FISH ; HYDROXYPROLINE ; ISOTHIOCYANATE
AbstractThe use of 2-(9-carbazole)ethyl chloroformate (CEOC) for pre-column derivatization of biogenic amines (BA) has been tested for the first time. The reagent reacts completely with BA within 3 min at ambient temperature in acetonitrile solution to form stable derivatives that are readily analyzed by reversed-phase HPLC. Study of the derivatization conditions revealed derivatization yields to be excellent in borate buffer over the pH range 9.0- 10.0. Maximum yields were obtained by use of a three- to fourfold molar excess of reagent. The reaction is extremely tolerant of common buffer salts, no decrease in reaction yield is discernible in well-buffered samples. The emission maximum for the CEOC-derivatives is 360 nm (lambda(ex) = 293 nm). All the derivatives fluoresced strongly and direct injection of the reaction mixture was possible, with no significant disturbance from the major fluorescent reagent degradation by-products, 2-(9-carbazole)ethanol (CEOC-OH) and bis-(2-(9-carbazole)ethyl) carbonate (CEOC)(2). Separation of the derivatized BA by high-performance liquid chromatography with gradient elution was tested on a Hypersil BDS C18 column. Excellent response linearity was observed over the concentration range from 0.25 to 94.6 mumol L-1 for the labeled BA. Detection limits were 117 - 840 fmol at a signal-to-noise ratio of 3: 1. Analysis of BA in a shrimp sauce extract was conducted to demonstrate the applicability of the technique to real sample matrixes; results were satisfactory.
Language英语
WOS IDWOS:000177132900008
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/139175
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
2.Qufu Normal Univ, Dept Chem, Qufu 273165, Shandong, Peoples R China
Recommended Citation
GB/T 7714
You, JM,Zhang, YK. Determination of biogenic Amines by RPHPLC with fluorescent detection after derivatization with 2-(9-carbazole)ethyl chloroformate (CEOC)[J]. CHROMATOGRAPHIA,2002,56(1-2):43-50.
APA You, JM,&Zhang, YK.(2002).Determination of biogenic Amines by RPHPLC with fluorescent detection after derivatization with 2-(9-carbazole)ethyl chloroformate (CEOC).CHROMATOGRAPHIA,56(1-2),43-50.
MLA You, JM,et al."Determination of biogenic Amines by RPHPLC with fluorescent detection after derivatization with 2-(9-carbazole)ethyl chloroformate (CEOC)".CHROMATOGRAPHIA 56.1-2(2002):43-50.
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