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题名: Determination of biogenic Amines by RPHPLC with fluorescent detection after derivatization with 2-(9-carbazole)ethyl chloroformate (CEOC)
作者: You, JM;  Zhang, YK
关键词: column liquid chromatography ;  fluorescent detection ;  2-(9-carbazole)ethyl chloroformate ;  biogenic amines
刊名: CHROMATOGRAPHIA
发表日期: 2002-07-01
卷: 56, 期:1-2, 页:43-50
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The use of 2-(9-carbazole)ethyl chloroformate (CEOC) for pre-column derivatization of biogenic amines (BA) has been tested for the first time. The reagent reacts completely with BA within 3 min at ambient temperature in acetonitrile solution to form stable derivatives that are readily analyzed by reversed-phase HPLC. Study of the derivatization conditions revealed derivatization yields to be excellent in borate buffer over the pH range 9.0- 10.0. Maximum yields were obtained by use of a three- to fourfold molar excess of reagent. The reaction is extremely tolerant of common buffer salts, no decrease in reaction yield is discernible in well-buffered samples. The emission maximum for the CEOC-derivatives is 360 nm (lambda(ex) = 293 nm). All the derivatives fluoresced strongly and direct injection of the reaction mixture was possible, with no significant disturbance from the major fluorescent reagent degradation by-products, 2-(9-carbazole)ethanol (CEOC-OH) and bis-(2-(9-carbazole)ethyl) carbonate (CEOC)(2). Separation of the derivatized BA by high-performance liquid chromatography with gradient elution was tested on a Hypersil BDS C18 column. Excellent response linearity was observed over the concentration range from 0.25 to 94.6 mumol L-1 for the labeled BA. Detection limits were 117 - 840 fmol at a signal-to-noise ratio of 3: 1. Analysis of BA in a shrimp sauce extract was conducted to demonstrate the applicability of the technique to real sample matrixes; results were satisfactory.
关键词[WOS]: LIQUID-CHROMATOGRAPHIC DETERMINATION ;  AUTOMATED PRECOLUMN DERIVATIZATION ;  RP-HPLC ;  O-PHTHALDIALDEHYDE ;  CHLORIDE ;  ACIDS ;  FOODS ;  FISH ;  HYDROXYPROLINE ;  ISOTHIOCYANATE
语种: 英语
WOS记录号: WOS:000177132900008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139175
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
2.Qufu Normal Univ, Dept Chem, Qufu 273165, Shandong, Peoples R China

Recommended Citation:
You, JM,Zhang, YK. Determination of biogenic Amines by RPHPLC with fluorescent detection after derivatization with 2-(9-carbazole)ethyl chloroformate (CEOC)[J]. CHROMATOGRAPHIA,2002,56(1-2):43-50.
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