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High-performance affinity chromatography for characterization of human immunoglobulin G digestion with papain
Luo, QZ; Mao, XQ; Kong, L; Huang, XD; Zou, HF
关键词Monolithic Rods Immunoglobulin g Papain
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2002-09-05
776期:2页:139-147
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]CO-ETHYLENE DIMETHACRYLATE) ; REVERSED-PHASE CHROMATOGRAPHY ; PROTEIN-A ; EXCHANGE CHROMATOGRAPHY ; F(AB')2 FRAGMENTS ; SEPARATION MEDIUM ; STATIONARY-PHASE ; PURIFICATION ; METHACRYLATE ; MEMBRANES
英文摘要Reactive continuous rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) were prepared within the confines of a stainless steel column. Then papain was immobilized on these monoliths either directly or linked by a spacer arm. In a further step, a protein A affinity column was used for the characterization of the digestion products of human immunoglobulin G (IgG) by papain. The results showed that papain immobilized on the monolithic rod through a spacer arm exhibits higher activity for the digestion of human IgG than that without a spacer arm. The apparent Michaelis-Menten kinetic constants of free and immobilized papain, K-m and V-max, were determined. The digestion conditions of human IgG with free and immobilized papain were optimized. Comparison of the thermal stability of free and immobilized papain showed that the immobilized papain exhibited higher thermal stability than the free enzyme. The half-time of immobilized papain reaches about a week under optimum pH and temperature conditions. (C) 2002 Elsevier Science B.V. All rights reserved.
语种英语
WOS记录号WOS:000177451900001
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被引频次:33[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/139180
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
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Luo, QZ,Mao, XQ,Kong, L,et al. High-performance affinity chromatography for characterization of human immunoglobulin G digestion with papain[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2002,776(2):139-147.
APA Luo, QZ,Mao, XQ,Kong, L,Huang, XD,&Zou, HF.(2002).High-performance affinity chromatography for characterization of human immunoglobulin G digestion with papain.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,776(2),139-147.
MLA Luo, QZ,et al."High-performance affinity chromatography for characterization of human immunoglobulin G digestion with papain".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 776.2(2002):139-147.
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