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题名: High-performance affinity chromatography for characterization of human immunoglobulin G digestion with papain
作者: Luo, QZ;  Mao, XQ;  Kong, L;  Huang, XD;  Zou, HF
关键词: monolithic rods ;  immunoglobulin G ;  papain
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2002-09-05
卷: 776, 期:2, 页:139-147
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Reactive continuous rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) were prepared within the confines of a stainless steel column. Then papain was immobilized on these monoliths either directly or linked by a spacer arm. In a further step, a protein A affinity column was used for the characterization of the digestion products of human immunoglobulin G (IgG) by papain. The results showed that papain immobilized on the monolithic rod through a spacer arm exhibits higher activity for the digestion of human IgG than that without a spacer arm. The apparent Michaelis-Menten kinetic constants of free and immobilized papain, K-m and V-max, were determined. The digestion conditions of human IgG with free and immobilized papain were optimized. Comparison of the thermal stability of free and immobilized papain showed that the immobilized papain exhibited higher thermal stability than the free enzyme. The half-time of immobilized papain reaches about a week under optimum pH and temperature conditions. (C) 2002 Elsevier Science B.V. All rights reserved.
关键词[WOS]: CO-ETHYLENE DIMETHACRYLATE) ;  REVERSED-PHASE CHROMATOGRAPHY ;  PROTEIN-A ;  EXCHANGE CHROMATOGRAPHY ;  F(AB')2 FRAGMENTS ;  SEPARATION MEDIUM ;  STATIONARY-PHASE ;  PURIFICATION ;  METHACRYLATE ;  MEMBRANES
语种: 英语
WOS记录号: WOS:000177451900001
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139180
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Luo, QZ,Mao, XQ,Kong, L,et al. High-performance affinity chromatography for characterization of human immunoglobulin G digestion with papain[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2002,776(2):139-147.
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