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High-performance affinity chromatography for characterization of human immunoglobulin G digestion with papain
Luo, QZ; Mao, XQ; Kong, L; Huang, XD; Zou, HF
KeywordMonolithic Rods Immunoglobulin g Papain
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2002-09-05
Volume776Issue:2Pages:139-147
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordCO-ETHYLENE DIMETHACRYLATE) ; REVERSED-PHASE CHROMATOGRAPHY ; PROTEIN-A ; EXCHANGE CHROMATOGRAPHY ; F(AB')2 FRAGMENTS ; SEPARATION MEDIUM ; STATIONARY-PHASE ; PURIFICATION ; METHACRYLATE ; MEMBRANES
AbstractReactive continuous rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) were prepared within the confines of a stainless steel column. Then papain was immobilized on these monoliths either directly or linked by a spacer arm. In a further step, a protein A affinity column was used for the characterization of the digestion products of human immunoglobulin G (IgG) by papain. The results showed that papain immobilized on the monolithic rod through a spacer arm exhibits higher activity for the digestion of human IgG than that without a spacer arm. The apparent Michaelis-Menten kinetic constants of free and immobilized papain, K-m and V-max, were determined. The digestion conditions of human IgG with free and immobilized papain were optimized. Comparison of the thermal stability of free and immobilized papain showed that the immobilized papain exhibited higher thermal stability than the free enzyme. The half-time of immobilized papain reaches about a week under optimum pH and temperature conditions. (C) 2002 Elsevier Science B.V. All rights reserved.
Language英语
WOS IDWOS:000177451900001
Citation statistics
Cited Times:33[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/139180
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Luo, QZ,Mao, XQ,Kong, L,et al. High-performance affinity chromatography for characterization of human immunoglobulin G digestion with papain[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2002,776(2):139-147.
APA Luo, QZ,Mao, XQ,Kong, L,Huang, XD,&Zou, HF.(2002).High-performance affinity chromatography for characterization of human immunoglobulin G digestion with papain.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,776(2),139-147.
MLA Luo, QZ,et al."High-performance affinity chromatography for characterization of human immunoglobulin G digestion with papain".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 776.2(2002):139-147.
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