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题名: Enantiomer separation of amino acids on microchip-based electrophoresis
作者: Wang, H;  Dai, ZP;  Wang, L;  Bai, JL;  Lin, BC
关键词: microchip-based electrophoresis ;  cyclodextrin ;  micellar electrokinetic capillary chromatography ;  chiral separation
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2002-06-01
卷: 30, 期:6, 页:665-669
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: The chiral separation 4 three amino acids labeled with fluorescein 5-isothiocyanate (FITC) was demonstrated on glass chip using cyclodextrin (CD)-sodiam dodecyl sulfate (SDS)-micellar electrokinetic, capillary chromatography (MEKC) and laser-induced fluorescence (LIF) detection. The effect of types and concentration of CD, concentration of SDS, and different Additives on chiral separation of amino acids was investigated. gamma-CD was, found to be effective in this respect. A buffer system consisting of 10 mmol/L borax (pH 10.0), 30 mmol/L of SDS, and 5 mmol/L gamma-CD as chiral selector provided good chiral separation of FITC-amino acid enantiomers tested.
关键词[WOS]: CAPILLARY-ELECTROPHORESIS ;  CHROMATOGRAPHY ;  CYCLODEXTRIN
语种: 英语
WOS记录号: WOS:000176657600007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139234
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Biochem Separat & Anal Grp, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China

Recommended Citation:
Wang, H,Dai, ZP,Wang, L,et al. Enantiomer separation of amino acids on microchip-based electrophoresis[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2002,30(6):665-669.
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