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题名: Analysis of chicken and turkey ovalburnins by microchip electrophoresis combined with exoglycosidase digestion
作者: Mao, XL;  Wang, K;  Du, YG;  Lin, BC
关键词: chicken ovalbumin ;  glycopeptides ;  microchip electrophoresis ;  miniaturization ;  peptide mapping ;  Turkey ovalburnin
刊名: ELECTROPHORESIS
发表日期: 2003-09-01
DOI: 10.1002/elps.200305598
卷: 24, 期:18, 页:3273-3278
收录类别: ISTP ;  SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The polypeptide and carbohydrate patterns of two glycoproteins, chicken ovalbumin (CO) and turkey ovalbumin (TO), were analyzed by microchip electrophoresis (ME), following digestion with proteases and exoglycosidases. Glycopeptides derived from ovalbumin were obtained by digestion with Pronase, followed by dialysis, and then separated by ME. Using CO as model, the method was developed to deduce the structure of glycans from glycoproteins by comparing the electropherograms of glycopeptides with and without digestion of exolycosidases. Applying the same approach, the structure of oligosaccharides linked to TO was determined. TO was found to contain high-mannose type oligosaccharides and oligosaccharides with terminal N-acetylglucosamine residues. The complete primary analysis of CO and TO by ME described in this paper provides a basis for an analysis of glycoproteins with an integrated microfluidic chip.
关键词[WOS]: CAPILLARY-ELECTROPHORESIS ;  MASS-SPECTROMETRY ;  OVALBUMIN ;  GLYCOSYLATION ;  GLYCOPROTEINS ;  OLIGOSACCHARIDES ;  PROTEOMICS ;  SEQUENCE ;  SYSTEM ;  CHIP
语种: 英语
WOS记录号: WOS:000185897100026
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139241
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Shanghai Acad Life Sci, Inst Biochem & Cell Biol, Beijing 100864, Peoples R China

Recommended Citation:
Mao, XL,Wang, K,Du, YG,et al. Analysis of chicken and turkey ovalburnins by microchip electrophoresis combined with exoglycosidase digestion[J]. ELECTROPHORESIS,2003,24(18):3273-3278.
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