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Analysis of chicken and turkey ovalburnins by microchip electrophoresis combined with exoglycosidase digestion
Mao, XL; Wang, K; Du, YG; Lin, BC
关键词Chicken Ovalbumin Glycopeptides Microchip Electrophoresis Miniaturization Peptide Mapping Turkey Ovalburnin
刊名ELECTROPHORESIS
2003-09-01
DOI10.1002/elps.200305598
24期:18页:3273-3278
收录类别ISTP ; SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]CAPILLARY-ELECTROPHORESIS ; MASS-SPECTROMETRY ; OVALBUMIN ; GLYCOSYLATION ; GLYCOPROTEINS ; OLIGOSACCHARIDES ; PROTEOMICS ; SEQUENCE ; SYSTEM ; CHIP
英文摘要The polypeptide and carbohydrate patterns of two glycoproteins, chicken ovalbumin (CO) and turkey ovalbumin (TO), were analyzed by microchip electrophoresis (ME), following digestion with proteases and exoglycosidases. Glycopeptides derived from ovalbumin were obtained by digestion with Pronase, followed by dialysis, and then separated by ME. Using CO as model, the method was developed to deduce the structure of glycans from glycoproteins by comparing the electropherograms of glycopeptides with and without digestion of exolycosidases. Applying the same approach, the structure of oligosaccharides linked to TO was determined. TO was found to contain high-mannose type oligosaccharides and oligosaccharides with terminal N-acetylglucosamine residues. The complete primary analysis of CO and TO by ME described in this paper provides a basis for an analysis of glycoproteins with an integrated microfluidic chip.
语种英语
WOS记录号WOS:000185897100026
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被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/139241
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Shanghai Acad Life Sci, Inst Biochem & Cell Biol, Beijing 100864, Peoples R China
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GB/T 7714
Mao, XL,Wang, K,Du, YG,et al. Analysis of chicken and turkey ovalburnins by microchip electrophoresis combined with exoglycosidase digestion[J]. ELECTROPHORESIS,2003,24(18):3273-3278.
APA Mao, XL,Wang, K,Du, YG,&Lin, BC.(2003).Analysis of chicken and turkey ovalburnins by microchip electrophoresis combined with exoglycosidase digestion.ELECTROPHORESIS,24(18),3273-3278.
MLA Mao, XL,et al."Analysis of chicken and turkey ovalburnins by microchip electrophoresis combined with exoglycosidase digestion".ELECTROPHORESIS 24.18(2003):3273-3278.
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