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题名: Peptide separation in hydrophilic interaction capillary electrochromatography
作者: Fu, HJ;  Jin, WH;  Xiao, H;  Huang, HW;  Zou, HF
关键词: capillary electrochromatography ;  hydrophilic interaction ;  peptides ;  separation mechanism ;  strong-cation exchange
刊名: ELECTROPHORESIS
发表日期: 2003-06-01
DOI: 10.1002/elps.200305462
卷: 24, 期:12-13, 页:2084-2091
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Separation of small peptides by hydrophilic interaction capillary electrochromatography (HI-CEC) has been investigated. The negative surface charge of a hydrophilic, strong-cation-exchange stationary phase (PolySULFOETHYL A) provided a substantial cathodic electroosmotic flow (EOF). The influence of acetonitrile content, ionic strength, mobile phase pH as well as applied voltage on the migration of the peptides was studied. Possible retention mechanisms of the peptides in HI-CEC were discussed. It was found that hydrophilic interaction between the solutes and the stationary phase played a major role in this system, especially when mobile phases with high acetonitrile content were used. However, an ion-exchange mechanism and electrophoretic mobility also affect the migration of the peptides in HI-CEC. Elution order and selectivity was proved to be different in HI-CEC and capillary zone electrophoresis (CZE), thus revealing the potential of HI-CEC as a complementary technique to CZE for the separation of peptides. Efficiency and selectivity of HI-CEC for the separation of peptides were demonstrated by baseline separating nine peptides in 6 min.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  REVERSED-PHASE ;  POLAR COMPOUNDS ;  ELECTROENDOOSMOTIC CHROMATOGRAPHY ;  EXCHANGE CHROMATOGRAPHY ;  NUCLEIC-ACIDS ;  PROTEINS ;  COLUMNS ;  BEHAVIOR ;  DRIVEN
语种: 英语
WOS记录号: WOS:000184233300017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139274
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Fu, HJ,Jin, WH,Xiao, H,et al. Peptide separation in hydrophilic interaction capillary electrochromatography[J]. ELECTROPHORESIS,2003,24(12-13):2084-2091.
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