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题名: Determination of basic pharmaceuticals in human serum by hydrophilic interaction capillary electrochromatography
作者: Fu, HJ;  Jin, WH;  Xiao, H;  Xie, CH;  Guo, BC;  Zou, HF
关键词: basic pharmaceuticals ;  capillary electrochromatography ;  human serum ;  hydrophilic interaction ;  strong-cation exchange
刊名: ELECTROPHORESIS
发表日期: 2004-02-01
卷: 25, 期:4-5, 页:600-606
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Hydrophilic interaction capillary electrochromatography (HI-CEC) for the determination of basic pharmaceuticals spiked in human serum is described. The organic modifier content, ionic strength, and pH value of the mobile phase as well as the applied voltage are optimized for separation and elution of these drug analytes. Excellent separation was achieved for drugs using a mobile phase composition of 80% v/v acetonitrile in 100 mm triethylamine phosphate (TEAP) buffer at pH 2.8 with column efficiencies for analytes more than 200 000 plates/m. The samples of human serum spiked with basic drugs were directly injected after a simple acetonitrile treatment. The linear range and reproducibility of these basic drugs using an external and internal standard method were compared. As a result, the reproducibility could be greatly improved by using the internal standard method. Good calibration curves with regression coefficients more than 0.998 in the range of 5-160 mug/mL were observed with the internal standard method. The limits of quantitation, based on standards with acceptable relative standard deviations (RSDs), were below 5 mug/mL. The intra- and inter-day precisions, determined as RSDs, were less than 4.57%.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  REVERSED-PHASE ;  STATIONARY PHASES ;  POLAR COMPOUNDS ;  SEPARATION ;  ELECTROPHORESIS ;  SILICA ;  DRUGS ;  IBUPROFEN ;  BEHAVIOR
语种: 英语
WOS记录号: WOS:000220046200009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139327
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Fu, HJ,Jin, WH,Xiao, H,et al. Determination of basic pharmaceuticals in human serum by hydrophilic interaction capillary electrochromatography[J]. ELECTROPHORESIS,2004,25(4-5):600-606.
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