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题名: Influence of moderate Joule heating on electroosmotic flow velocity, retention, and efficiency in capillary electrochromatography
作者: Chen, GF;  Tallarek, U;  Seidel-Morgenstern, A;  Zhang, YK
关键词: Joule heating ;  electroosmotic flow ;  retention factor ;  efficiency ;  benzenes ;  benzoates
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2004-07-30
DOI: 10.1016/j.chroma.2004.05.054
卷: 1044, 期:1-2, 页:287-294
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The influence of Joule heating on electroosmotic flow velocity, the retention factor of neutral analytes, and separation efficiency in capillary electrochromatography was investigated theoretically and experimentally. A plot of electrical current against the applied electrical field strength was used to evaluate the Joule heating effect. When the mobile phase concentration of Tris buffer exceeded 5.0 mM in the studied capillary electrochromatography systems using particulate and monolithic columns (with an accompanying power level of heat dissipation higher than 0.35 W/m), the Joule heating effect became clearly noticeable. Theoretical models for describing the variation of electroosmotic flow velocity with increasing applied field strength and the change of retention factors for neutral analytes with electrical field strength at higher Tris buffer concentrations were analyzed to explain consequences of Joule heating in capillary electrochromatography. Qualitative agreement between experimental data and implications of the theoretical model analysis was observed. The decrease of separation efficiency in capillary electrochromatography with macroporous octadecylsilica particles at high buffer concentration can be also attributed to Joule heating mainly via the increased axial diffusion of the analyte molecules and dispersion of solute bands by a nonuniform electroosmotic flow profile over the column cross-section. However, within a moderate temperature range, the contribution of the macroscopic velocity profile in the column arising from radial temperature gradients is insignificant. (C) 2004 Elsevier B.V. All rights reserved.
关键词[WOS]: MICELLAR ELECTROKINETIC CHROMATOGRAPHY ;  HIGH BUFFER CONCENTRATIONS ;  ELECTRIC-FIELD STRENGTHS ;  ZONE-ELECTROPHORESIS ;  TEMPERATURE-GRADIENTS ;  PACKED CAPILLARIES ;  PLATE HEIGHT ;  PORE FLOW ;  DISPERSION ;  SEPARATION
语种: 英语
WOS记录号: WOS:000222984300026
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139330
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
2.Univ Magdeburg, Inst Verfahrenstech, D-39106 Magdeburg, Germany

Recommended Citation:
Chen, GF,Tallarek, U,Seidel-Morgenstern, A,et al. Influence of moderate Joule heating on electroosmotic flow velocity, retention, and efficiency in capillary electrochromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2004,1044(1-2):287-294.
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