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Preparation and time-resolved fluorometric application of luminescent europium nanoparticles
Tan, MQ; Ye, ZQ; Wang, GL; Yuan, JL
刊名CHEMISTRY OF MATERIALS
2004-06-15
DOI10.1021/cm030305n
16期:12页:2494-2498
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences ; Technology
类目[WOS]Chemistry, Physical ; Materials Science, Multidisciplinary
研究领域[WOS]Chemistry ; Materials Science
关键词[WOS]DOPED SILICA NANOPARTICLES ; PROSTATE-SPECIFIC ANTIGEN ; DNA HYBRIDIZATION ; ULTRASENSITIVE DETECTION ; BIOANALYTICAL ASSAYS ; FLUORESCENT CHELATE ; FLUOROIMMUNOASSAY ; IMMUNOASSAYS ; PROBES ; BIOMOLECULES
英文摘要Novel silica-coated luminescent europium (SCLE) nanoparticles have been prepared by copolymerization of tetraethyl orthosilicate and 3-aminopropyl(triethoxy)silane with ammonium hydroxide in a water-in-oil microemulsion containing a luminescent Eu3+ chelate, Triton X-100, n-octanol, and cyclohexane. The nanoparticles were characterized by transmission electron microscopy, X-ray diffraction, and UV-vis and luminescence spectra. The results show that the nanoparticles are spherical and uniform in size, 50 +/- 5 nm in diameter, and have high photostability and long luminescence lifetime (770 mus). Because primary amino groups have been directly introduced to the nanoparticle's surface by using 3-aminopropyl-(triethoxyl)silane in nanoparticle preparation, the surface modification and bioconjugation of the nanoparticles are easier and reproducible. The nanoparticles were used for streptavidin (SA) labeling, and the nanoparticle-labeled streptavidin was successfully used in time-resolved fluoroimmunoassay of human a-fetoprotein. The results reveal that the new nanoparticles are favorable for use as a new type of luminescence probe for highly sensitive time-resolved luminescence bioassay.
语种英语
WOS记录号WOS:000221976400023
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被引频次:92[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/139350
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116012, Peoples R China
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GB/T 7714
Tan, MQ,Ye, ZQ,Wang, GL,et al. Preparation and time-resolved fluorometric application of luminescent europium nanoparticles[J]. CHEMISTRY OF MATERIALS,2004,16(12):2494-2498.
APA Tan, MQ,Ye, ZQ,Wang, GL,&Yuan, JL.(2004).Preparation and time-resolved fluorometric application of luminescent europium nanoparticles.CHEMISTRY OF MATERIALS,16(12),2494-2498.
MLA Tan, MQ,et al."Preparation and time-resolved fluorometric application of luminescent europium nanoparticles".CHEMISTRY OF MATERIALS 16.12(2004):2494-2498.
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