中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Preparation and time-resolved fluorometric application of luminescent europium nanoparticles
作者: Tan, MQ;  Ye, ZQ;  Wang, GL;  Yuan, JL
刊名: CHEMISTRY OF MATERIALS
发表日期: 2004-06-15
DOI: 10.1021/cm030305n
卷: 16, 期:12, 页:2494-2498
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Technology
类目[WOS]: Chemistry, Physical ;  Materials Science, Multidisciplinary
研究领域[WOS]: Chemistry ;  Materials Science
英文摘要: Novel silica-coated luminescent europium (SCLE) nanoparticles have been prepared by copolymerization of tetraethyl orthosilicate and 3-aminopropyl(triethoxy)silane with ammonium hydroxide in a water-in-oil microemulsion containing a luminescent Eu3+ chelate, Triton X-100, n-octanol, and cyclohexane. The nanoparticles were characterized by transmission electron microscopy, X-ray diffraction, and UV-vis and luminescence spectra. The results show that the nanoparticles are spherical and uniform in size, 50 +/- 5 nm in diameter, and have high photostability and long luminescence lifetime (770 mus). Because primary amino groups have been directly introduced to the nanoparticle's surface by using 3-aminopropyl-(triethoxyl)silane in nanoparticle preparation, the surface modification and bioconjugation of the nanoparticles are easier and reproducible. The nanoparticles were used for streptavidin (SA) labeling, and the nanoparticle-labeled streptavidin was successfully used in time-resolved fluoroimmunoassay of human a-fetoprotein. The results reveal that the new nanoparticles are favorable for use as a new type of luminescence probe for highly sensitive time-resolved luminescence bioassay.
关键词[WOS]: DOPED SILICA NANOPARTICLES ;  PROSTATE-SPECIFIC ANTIGEN ;  DNA HYBRIDIZATION ;  ULTRASENSITIVE DETECTION ;  BIOANALYTICAL ASSAYS ;  FLUORESCENT CHELATE ;  FLUOROIMMUNOASSAY ;  IMMUNOASSAYS ;  PROBES ;  BIOMOLECULES
语种: 英语
WOS记录号: WOS:000221976400023
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139350
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116012, Peoples R China

Recommended Citation:
Tan, MQ,Ye, ZQ,Wang, GL,et al. Preparation and time-resolved fluorometric application of luminescent europium nanoparticles[J]. CHEMISTRY OF MATERIALS,2004,16(12):2494-2498.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Tan, MQ]'s Articles
 [Ye, ZQ]'s Articles
 [Wang, GL]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Tan, MQ]‘s Articles
 [Ye, ZQ]‘s Articles
 [Wang, GL]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace