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Novel fluorescent europium chelate-doped silica nanoparticles: preparation, characterization and time-resolved fluorometric application
Ye, ZQ; Tan, MQ; Wang, GL; Yuan, JL
刊名JOURNAL OF MATERIALS CHEMISTRY
2004-03-07
DOI10.1039/b311905j
14期:5页:851-856
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences ; Technology
类目[WOS]Chemistry, Physical ; Materials Science, Multidisciplinary
研究领域[WOS]Chemistry ; Materials Science
关键词[WOS]PROSTATE-SPECIFIC ANTIGEN ; BIOANALYTICAL ASSAYS ; REVERSE MICELLE ; FLUOROIMMUNOASSAY ; MICROEMULSION ; LABELS ; HYBRIDIZATION ; IMMUNOASSAYS
英文摘要Novel fluorescent europium(III) chelate-doped silica nanoparticles were prepared and characterized as a new type of fluorescence probe for quantitative bioassay. The preparation was carried Out in a water-in-oil (w/o) microemulsion consisting of a strongly fluorescent Eu3+ chelate, 4,4'-bis(1",1",1",2",2",3",3"-heptafluoro-4",6"-hexanedion-6"-yl)-o-terphenyl-Eu3+ (BHHT-EU3+), surfactant (Triton X-100), co-surfactant (n-hexanol, n-heptanol or n-octanol), aqueous phase (H2O or D2O) and oil phase (cyclohexane) by controlling the hydrolysis of tetraethylorthosilicate (TEOS). The effects of different co-surfactants and aqueous phases on the size and fluorescence lifetime of the nanoparticles were investigated. The results reveal that the size of the nanoparticles is decreased with a change of co-surfactants from n-hexanol to n-octanol, and the fluorescence lifetime of the nanoparticles is increased with a change of aqueous phase from H2O to D2O. A new method was established for the surface modification and bioconjugation of the nanoparticles. Nanoparticle-labeled streptavidin (SA) was used for the time-resolved fluoroimmunoassay of human hepatitis B surface antigen (HBsAg). The result shows that the new fluorescent europium(III) chelate-doped silica nanoparticles are suitable to be used as a fluorescence probe for highly sensitive bioassays.
语种英语
WOS记录号WOS:000220377500012
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被引频次:79[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/139351
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116012, Peoples R China
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Ye, ZQ,Tan, MQ,Wang, GL,et al. Novel fluorescent europium chelate-doped silica nanoparticles: preparation, characterization and time-resolved fluorometric application[J]. JOURNAL OF MATERIALS CHEMISTRY,2004,14(5):851-856.
APA Ye, ZQ,Tan, MQ,Wang, GL,&Yuan, JL.(2004).Novel fluorescent europium chelate-doped silica nanoparticles: preparation, characterization and time-resolved fluorometric application.JOURNAL OF MATERIALS CHEMISTRY,14(5),851-856.
MLA Ye, ZQ,et al."Novel fluorescent europium chelate-doped silica nanoparticles: preparation, characterization and time-resolved fluorometric application".JOURNAL OF MATERIALS CHEMISTRY 14.5(2004):851-856.
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