中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Novel fluorescent europium chelate-doped silica nanoparticles: preparation, characterization and time-resolved fluorometric application
作者: Ye, ZQ;  Tan, MQ;  Wang, GL;  Yuan, JL
刊名: JOURNAL OF MATERIALS CHEMISTRY
发表日期: 2004-03-07
DOI: 10.1039/b311905j
卷: 14, 期:5, 页:851-856
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Technology
类目[WOS]: Chemistry, Physical ;  Materials Science, Multidisciplinary
研究领域[WOS]: Chemistry ;  Materials Science
英文摘要: Novel fluorescent europium(III) chelate-doped silica nanoparticles were prepared and characterized as a new type of fluorescence probe for quantitative bioassay. The preparation was carried Out in a water-in-oil (w/o) microemulsion consisting of a strongly fluorescent Eu3+ chelate, 4,4'-bis(1",1",1",2",2",3",3"-heptafluoro-4",6"-hexanedion-6"-yl)-o-terphenyl-Eu3+ (BHHT-EU3+), surfactant (Triton X-100), co-surfactant (n-hexanol, n-heptanol or n-octanol), aqueous phase (H2O or D2O) and oil phase (cyclohexane) by controlling the hydrolysis of tetraethylorthosilicate (TEOS). The effects of different co-surfactants and aqueous phases on the size and fluorescence lifetime of the nanoparticles were investigated. The results reveal that the size of the nanoparticles is decreased with a change of co-surfactants from n-hexanol to n-octanol, and the fluorescence lifetime of the nanoparticles is increased with a change of aqueous phase from H2O to D2O. A new method was established for the surface modification and bioconjugation of the nanoparticles. Nanoparticle-labeled streptavidin (SA) was used for the time-resolved fluoroimmunoassay of human hepatitis B surface antigen (HBsAg). The result shows that the new fluorescent europium(III) chelate-doped silica nanoparticles are suitable to be used as a fluorescence probe for highly sensitive bioassays.
关键词[WOS]: PROSTATE-SPECIFIC ANTIGEN ;  BIOANALYTICAL ASSAYS ;  REVERSE MICELLE ;  FLUOROIMMUNOASSAY ;  MICROEMULSION ;  LABELS ;  HYBRIDIZATION ;  IMMUNOASSAYS
语种: 英语
WOS记录号: WOS:000220377500012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139351
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116012, Peoples R China

Recommended Citation:
Ye, ZQ,Tan, MQ,Wang, GL,et al. Novel fluorescent europium chelate-doped silica nanoparticles: preparation, characterization and time-resolved fluorometric application[J]. JOURNAL OF MATERIALS CHEMISTRY,2004,14(5):851-856.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Ye, ZQ]'s Articles
 [Tan, MQ]'s Articles
 [Wang, GL]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Ye, ZQ]‘s Articles
 [Tan, MQ]‘s Articles
 [Wang, GL]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace