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Study on human alpha-lactalbumin-calcium interaction using affinity capillary electrophoresis
Wang, C; Zheng, YF; Xiong, JH; Weng, QF; Yang, J; Yang, Q; Xu, GW
KeywordAffinity Capillary Electrophoresis Human Alpha-lactalbumin Calcium Binding
Source PublicationACTA CHIMICA SINICA
2004-02-14
Volume62Issue:3Pages:279-282
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordBIOMOLECULAR RECOGNITION ; BINDING CONSTANTS ; BOVINE ; PROTEIN
AbstractAffinity capillary electrophoresis was used to study the interaction of human alpha-lactalbumin (alpha-HLA) with calcium ion(Ca2+). With constant concentration of alpha-HLA as a receptor and various concentrations of Ca2+ as a ligand in the running buffer, changes in electrophoretic mobilities of alpha-HLA were observed when complexes of alpha-HLA and Ca2+ came into being. Based on Scatchard analysis of the mobility ratios (M) of alpha-HLA to Ca2+, it was found that the apparent binding constant (K-app) was 2.0 x 10(7) (mol/L)(-1). In addition, the effect of Ca2+ on the unfolding of alpha-HLA induced by urea and heating was studied, and the results showed that the binding of Ca2+ increased the stability of alpha-HLA against the action of denaturing agent (such as urea) and heating.
Language英语
WOS IDWOS:000188877700010
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/139365
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Wang, C,Zheng, YF,Xiong, JH,et al. Study on human alpha-lactalbumin-calcium interaction using affinity capillary electrophoresis[J]. ACTA CHIMICA SINICA,2004,62(3):279-282.
APA Wang, C.,Zheng, YF.,Xiong, JH.,Weng, QF.,Yang, J.,...&Xu, GW.(2004).Study on human alpha-lactalbumin-calcium interaction using affinity capillary electrophoresis.ACTA CHIMICA SINICA,62(3),279-282.
MLA Wang, C,et al."Study on human alpha-lactalbumin-calcium interaction using affinity capillary electrophoresis".ACTA CHIMICA SINICA 62.3(2004):279-282.
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