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题名: Study on human alpha-lactalbumin-calcium interaction using affinity capillary electrophoresis
作者: Wang, C;  Zheng, YF;  Xiong, JH;  Weng, QF;  Yang, J;  Yang, Q;  Xu, GW
关键词: affinity capillary electrophoresis ;  human alpha-lactalbumin ;  calcium binding
刊名: ACTA CHIMICA SINICA
发表日期: 2004-02-14
卷: 62, 期:3, 页:279-282
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: Affinity capillary electrophoresis was used to study the interaction of human alpha-lactalbumin (alpha-HLA) with calcium ion(Ca2+). With constant concentration of alpha-HLA as a receptor and various concentrations of Ca2+ as a ligand in the running buffer, changes in electrophoretic mobilities of alpha-HLA were observed when complexes of alpha-HLA and Ca2+ came into being. Based on Scatchard analysis of the mobility ratios (M) of alpha-HLA to Ca2+, it was found that the apparent binding constant (K-app) was 2.0 x 10(7) (mol/L)(-1). In addition, the effect of Ca2+ on the unfolding of alpha-HLA induced by urea and heating was studied, and the results showed that the binding of Ca2+ increased the stability of alpha-HLA against the action of denaturing agent (such as urea) and heating.
关键词[WOS]: BIOMOLECULAR RECOGNITION ;  BINDING CONSTANTS ;  BOVINE ;  PROTEIN
语种: 英语
WOS记录号: WOS:000188877700010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139365
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Wang, C,Zheng, YF,Xiong, JH,et al. Study on human alpha-lactalbumin-calcium interaction using affinity capillary electrophoresis[J]. ACTA CHIMICA SINICA,2004,62(3):279-282.
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