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题名: Native fluorescence detection of flavin denvatives by microchip capillary electrophoresis with laser-induced fluorescence intensified charge-coupled device detection
作者: Qin, JH;  Fung, YS;  Zhu, DR;  Lin, BC
关键词: chip technology ;  microfluidics ;  detection ;  electrophoresis ;  laser-induced fluorescence detection ;  riboflavin ;  flavins
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2004-02-20
DOI: 10.1016/j.chroma.2003.10.055
卷: 1027, 期:1-2, 页:223-229
收录类别: ISTP ;  SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: To widen the scope of laser-induced fluorescence (LIF) for detection in microchip capillary electrophoresis (CE), a microchip CE LIF-ICCD (intensified charge-coupled device) system based on a tunable wavelength dye laser pumped by a pico-second pulse nitrogen laser for excitation and a spectrograph with ICCD for detection had developed to demonstrate the enhancement in detection sensitivity by the following three approaches: direct detection of native fluorescence, improvement of signal-to-noise ratio by pulse laser excitation and time delay detection, and selective spectral acquisition by multi-channel detection. Riboflavin, flavin mononucleotide (FMN) and flavin-adenine dinucleotide (FAD) have been selected as they are dietetically important and microchip CE provides a promising onsite detection method. The results indicate a strong effect of wavelength on detection sensitivity and the need to tune wavelength for direct detection. Under optimized conditions (excitation 450 urn, emission 520 nm, gate delay time 45 ns, 20 mM phosphate buffer at pH 7. 1), the following results were obtained under static condition: Working ranges (0.6-350 mug/l, r > 0.99), detection limits (0.15-1.0 mug/l) and peak height repeatability (1.8-2.2% R.S.D.), all within the applicability range for body fluids or beverages such as human urine and cow milk. Baseline separation of three flavins was obtained under dynamic condition and the fluorescence spectra acquired assist the identification of alkaline-degraded products of riboflavin. Thus, the capability to check peak purity and identify unknown peaks has been demonstrated. (C) 2003 Elsevier B.V. All rights reserved.
关键词[WOS]: ADENINE-DINUCLEOTIDE ;  ZONE-ELECTROPHORESIS ;  MICROFLUIDIC CHANNELS ;  GLASS CHIPS ;  RIBOFLAVIN ;  MONONUCLEOTIDE ;  QUANTITATION ;  MICRODEVICE ;  INTEGRATION ;  STACKING
语种: 英语
WOS记录号: WOS:000188498900031
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139376
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Qin, JH,Fung, YS,Zhu, DR,et al. Native fluorescence detection of flavin denvatives by microchip capillary electrophoresis with laser-induced fluorescence intensified charge-coupled device detection[J]. JOURNAL OF CHROMATOGRAPHY A,2004,1027(1-2):223-229.
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