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Construction of two-dimensional separation platform of coupling capillary isoelectric focusing with capillary non-gel sieving electrophoresis for proteins
Liu, HC; Yang, C; Yang, Q; Zhang, WB; Zhang, YK
关键词Hollow Fiber Interface Two-dimensional Separation Platform Capillary Isoelectric Focusing Electrophoresis Capillary Non-gel Sieving Electrophoresis
刊名CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2004-03-01
32期:3页:273-277
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]CHROMATOGRAPHY ; PROTEOMICS
英文摘要A novel dialysis hollow fiber interface was designed and fabricated and a two-dimensional separation platform of coupling capillary isoelectric focusing (CIEF) with capillary non-gel sieving electrophoresis (CNGE) for proteins was constructed by the interface. Classic two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) was transferred to the capillary format via the interface. The hemoglobin (Hb) sample was selected to demonstrate the 2-D separation system's feasibility and resolving power. The 2-D separation system was found to greatly increase the resolving power and overall peak capacity over those obtained for either dimension alone.
语种英语
WOS记录号WOS:000220700500001
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被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/139383
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
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GB/T 7714
Liu, HC,Yang, C,Yang, Q,et al. Construction of two-dimensional separation platform of coupling capillary isoelectric focusing with capillary non-gel sieving electrophoresis for proteins[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2004,32(3):273-277.
APA Liu, HC,Yang, C,Yang, Q,Zhang, WB,&Zhang, YK.(2004).Construction of two-dimensional separation platform of coupling capillary isoelectric focusing with capillary non-gel sieving electrophoresis for proteins.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,32(3),273-277.
MLA Liu, HC,et al."Construction of two-dimensional separation platform of coupling capillary isoelectric focusing with capillary non-gel sieving electrophoresis for proteins".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 32.3(2004):273-277.
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