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题名: Chemical method of breaking the cell-loaded sodium alginate/chitosan microcapsules
作者: Xue, WM;  Yu, WT;  Liu, XD;  He, X;  Wang, W;  Ma, XJ
关键词: chitosan ;  sodium alginate ;  microcapsule ;  breaking of microcapsule ;  cell activity
刊名: CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
发表日期: 2004-07-01
卷: 25, 期:7, 页:1342-1346
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: A novel chemical method to break and dissolve cell-loaded microcapsules under physiological condition was established, especially aiming at alginate/chitosan/alginate (ACA) microcapsules. The microcapsule-broken process was designed to proceed under an approximately physiological condition for the consideration of maintaining the cytoactive. The microbial cell (Pichia pastoris GS115) and animal cell (L929) were chosen as the model cells, and the microcapsule-broken solution (MBS) was basically composed of NaHCO3 and Na3C6H5O7 . 2H(2)O. The effects of MBS on the breaking of microcapsules and on the cytoactive were thoroughly investigated. Moreover, the mechanism of the breaking of microcapsules was analyzed. It was demonstrated that the microcapsules could be broken within 30 s, the breaking ratio of the microcapsule was 100%, and the fragments of the microcapsules was dissolved completely. It was also determined that the survival rate of cells was above 85%. Thus, the novel method will make it possible to quantitatively analyze the growth of cells in microcapsules, to examine the morphology and function of cells, to recover the cells and to purify metabolic products, which is always difficult to realize at present. Therefore, the chemical method of breaking and dissolving cell-loaded microcapsules would be the important component of microencapsulation technology applied to the cell culture and cell transplantation.
语种: 英语
WOS记录号: WOS:000222906300039
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139388
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.NW Univ Xian, Coll Chem Engn, Xian 710069, Peoples R China
3.Dalian Med Univ, Coll Lab Med, Dalian 116027, Peoples R China

Recommended Citation:
Xue, WM,Yu, WT,Liu, XD,et al. Chemical method of breaking the cell-loaded sodium alginate/chitosan microcapsules[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2004,25(7):1342-1346.
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