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LC-MS/MS determination of naringin, hesperidin and neohesperidin in rat serum after orally administrating the decoction of Bulpleurum falcatum L. and Fractus aurantii
Li, XL; Xiao, HB; Liang, XM; Shi, DZ; Liu, JG
KeywordLc-ms/ms Hesperidin Naringin Neohesperidin Qualification Quantification
Source PublicationJOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
2004-01-27
DOI10.1016/j.japna.2003.08.002
Volume34Issue:1Pages:159-166
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Life Sciences & Biomedicine
WOS SubjectChemistry, Analytical ; Pharmacology & Pharmacy
WOS Research AreaChemistry ; Pharmacology & Pharmacy
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; GRAPEFRUIT JUICE ; HUMAN URINE ; HPLC
AbstractTo identify and quantify biologically active components in rat serum after orally administrating the decoction of Bulpleurum falcatum L. and Fractus aurantii, one of traditional Chinese medicines (TCMs), high-performance liquid chromatography (HPLC)-tandem mass spectrometry method was developed and validated. The HPLC separation was carried out on a Waters Nova Pak C-18 column using acetonitrile and water as mobile phase after the sample of rat serum was cleaned up with solid-phase extraction. Atmospheric pressure chemical ionization in the negative ion mode and selected reaction monitoring (SRM) method was developed to determine the active components. Three flavonoids of hesperidin, neohesperidin and naringin were identified in the serum by comparing their retention times and three independent SRM precursor/product ion transitions with those of corresponding reference standards. The concentrations of naringin, hesperidin and neohesperidin in rat serum determined by SRM measurement were 16.3, 11.9 and 14.3 ng/ml, respectively, after orally administrating the decoction of B. falcatum L. and F. aurantii. This method was validated in terms of recovery, linearity, accuracy and precision (intra- and interday variation). The recoveries from spiked control samples were 93.0, 89.3 and 91.2% for hesperidin, neohesperidin and naringin, respectively. Linearity in rat serum was observed over the range of 2.0-50.0 ng/ml. Percent bias (accuracy) and precision were well within the acceptable range and the relative standard deviation (R.S.D.) of the measured rat serum samples was less than 10% (n = 5). (C) 2003 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000189093400018
Citation statistics
Cited Times:47[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/139391
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China
2.Xiyuan Hosp, Chinese Acad Tradit Chinese Med, Beijing 100091, Peoples R China
Recommended Citation
GB/T 7714
Li, XL,Xiao, HB,Liang, XM,et al. LC-MS/MS determination of naringin, hesperidin and neohesperidin in rat serum after orally administrating the decoction of Bulpleurum falcatum L. and Fractus aurantii[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2004,34(1):159-166.
APA Li, XL,Xiao, HB,Liang, XM,Shi, DZ,&Liu, JG.(2004).LC-MS/MS determination of naringin, hesperidin and neohesperidin in rat serum after orally administrating the decoction of Bulpleurum falcatum L. and Fractus aurantii.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,34(1),159-166.
MLA Li, XL,et al."LC-MS/MS determination of naringin, hesperidin and neohesperidin in rat serum after orally administrating the decoction of Bulpleurum falcatum L. and Fractus aurantii".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 34.1(2004):159-166.
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