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题名: THE PURIFICATION OF RECOMBINANT HEPATITIS B SURFACE ANTIGEN BY HOLLOW FIBRE ULTRAFILTRATION MEMBRANE DIAFILTRATION
作者: Chai, Xi-Jun1;  Lin, Yi-Zheng1;  Qin, Jian-Jun1;  Jiang, Cheng-Zhang1
刊名: BIOTECHNOLOGY TECHNIQUES
发表日期: 1992-07-01
卷: 6, 期:4, 页:381-384
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods ;  Biotechnology & Applied Microbiology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology
英文摘要: Recombinant hepatitis B surface antigen (HBsAg) broth was purified by diafiltration with a hollow fibre ultrafiltration membrane (cut off 100kDa), by pre-treating the HBsAg broth with enzyme, most of the contaminating proteins was removed and very high recovery ratio of HBsAg was achieved. When HBsAg solution was concentrated using hollow fibre ultrafiltration membrane to 10% of its original volume, the HBsAg was recovered almost completely. Accordingly, membrane purification of HBsAg is a high yield, fast method.
语种: 英语
WOS记录号: WOS:000207027400018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139410
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Membrane Proc, Dalian 116012, Peoples R China

Recommended Citation:
Chai, Xi-Jun,Lin, Yi-Zheng,Qin, Jian-Jun,et al. THE PURIFICATION OF RECOMBINANT HEPATITIS B SURFACE ANTIGEN BY HOLLOW FIBRE ULTRAFILTRATION MEMBRANE DIAFILTRATION[J]. BIOTECHNOLOGY TECHNIQUES,1992,6(4):381-384.
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