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题名: Development and performance of laser-induced fluorescence microfluidic chip analyzer
作者: Zhou, XM;  Dai, ZP;  Lin, BC
关键词: laser-induced fluorescence spectrometer ;  microfluidic chip ;  microfluidic chip analyzer
刊名: CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
发表日期: 2004-03-10
卷: 25, 期:3, 页:436-440
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: A laser-induced fluorescence microfluidic chip system was developed. The detection system was designed according to the confocal principle. Main features of the system include the alternated emission filter in order to select many dyes, and computer controlled focusing via 3-D micro-manipulator and charge coupled device (CCD), and two curves of the current vs. time and two curves of the voltage vs. time for injection channel and separation channel displayed on the computer's screen. The detection limit reached 6. 67 X 10(-13) mol/L for rhodamine 6G. The linear range of detection was 4.02 X 10(-6) mol/Lsimilar to4.02 X 10(-9) mol/L. The high performance of the system was demonstrated by separation and detection of DNA fragments and PCR products on a home-made cast-molded PMMA microchip.
关键词[WOS]: CHEMILUMINESCENCE DETECTION ;  FABRICATION ;  SYSTEMS
语种: 英语
WOS记录号: WOS:000220526000010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139468
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 11063, Peoples R China

Recommended Citation:
Zhou, XM,Dai, ZP,Lin, BC. Development and performance of laser-induced fluorescence microfluidic chip analyzer[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2004,25(3):436-440.
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