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Development and performance of laser-induced fluorescence microfluidic chip analyzer
Zhou, XM; Dai, ZP; Lin, BC
关键词Laser-induced Fluorescence Spectrometer Microfluidic Chip Microfluidic Chip Analyzer
刊名CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
2004-03-10
25期:3页:436-440
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]CHEMILUMINESCENCE DETECTION ; FABRICATION ; SYSTEMS
英文摘要A laser-induced fluorescence microfluidic chip system was developed. The detection system was designed according to the confocal principle. Main features of the system include the alternated emission filter in order to select many dyes, and computer controlled focusing via 3-D micro-manipulator and charge coupled device (CCD), and two curves of the current vs. time and two curves of the voltage vs. time for injection channel and separation channel displayed on the computer's screen. The detection limit reached 6. 67 X 10(-13) mol/L for rhodamine 6G. The linear range of detection was 4.02 X 10(-6) mol/Lsimilar to4.02 X 10(-9) mol/L. The high performance of the system was demonstrated by separation and detection of DNA fragments and PCR products on a home-made cast-molded PMMA microchip.
语种英语
WOS记录号WOS:000220526000010
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被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/139468
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 11063, Peoples R China
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GB/T 7714
Zhou, XM,Dai, ZP,Lin, BC. Development and performance of laser-induced fluorescence microfluidic chip analyzer[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2004,25(3):436-440.
APA Zhou, XM,Dai, ZP,&Lin, BC.(2004).Development and performance of laser-induced fluorescence microfluidic chip analyzer.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,25(3),436-440.
MLA Zhou, XM,et al."Development and performance of laser-induced fluorescence microfluidic chip analyzer".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 25.3(2004):436-440.
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