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题名: Fluorescent-based single-strand conformation polymorphism/heteroduplex capillary electrophoretic mutation analysis of the p53 gene
作者: Zhao, CX;  Xu, GW;  Shi, XZ;  Ma, JM;  Zhang, Y;  Lv, S;  Weng, QF;  Yang, Q
刊名: ANALYTICAL SCIENCES
发表日期: 2004-07-01
卷: 20, 期:7, 页:1001-1005
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Fluorescent-based single-strand conformation polymorphism (F-SSCP) analysis with capillary electrophoresis (CE) is the most common method for the detection of mutation because of its high sensitivity and resolution. In this study, we prepared an inexpensive linear polyacrylamide (LPA), and successfully applied it to CE-SSCP analysis and tandem CESSCP/heteroduplex analysis (HA) of the P53 gene on an ABI capillary genetic analyzer. A comparison of the sieving capabilities of a homemade LPA and commercial polydimethylacrylamide (PDMA) demonstrates that the homemade LPA has a higher resolution, a shorter analysis time, and is more suitable for tandem SSCP/HA than commercial PDMA. To show the usefulness, mutations of P53 gene exon 7 - 8 in 37 tumor samples were investigated by using homemade LPA. The results indicate that 10 mutations were found in 9 of 37 cases; the majority of P53 mutations were missense mutations, and 70% were located in exon 7, which plays an important role in neoplastic progression in human tumorigenesis.
关键词[WOS]: POLYMORPHISM ANALYSIS ;  POLYMER-SOLUTIONS ;  HIGH-THROUGHPUT ;  DNA ;  SEPARATION
语种: 英语
WOS记录号: WOS:000222697500002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139487
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromtog R&A, Dalian 116011, Peoples R China
2.Dalian Med Univ, Secondary Affiliated Hosp, Dalian 116027, Peoples R China

Recommended Citation:
Zhao, CX,Xu, GW,Shi, XZ,et al. Fluorescent-based single-strand conformation polymorphism/heteroduplex capillary electrophoretic mutation analysis of the p53 gene[J]. ANALYTICAL SCIENCES,2004,20(7):1001-1005.
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