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题名: Modeling, by frontal analysis, of the adsorption of bovine serum albumin on cibacron blue-modified cellulose membranes
作者: Hao, WQ;  Wang, JD;  Li, JH
关键词: membrane-affinity chromatography ;  frontal analysis ;  cibacron blue F3GA ;  bovine serum albumin
刊名: CHROMATOGRAPHIA
发表日期: 2004-10-01
DOI: 10.1365/s10337-004-0398-2
卷: 60, 期:7-8, 页:449-454
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The adsorption of bovine serum albumin (BSA) on cibacron blue F3GA cellulose affinity membranes has been studied by frontal analysis. The ideal stirred tank (IST) model, the modified stirred tank (MST) model, and the equilibrium-dispersive (E-D) model were used to describe experimental breakthrough curves. The E-D model was found to best describe the initial region of the breakthrough curve whereas the MST model was best for the late region, implying a difference between the initial and late stages of adsorption. According to the E-D model, the effect of axial dispersion was not negligible, possibly because of experimental factors such as inhomogeneous distribution of the feed solution and the thinness of affinity membranes. The variable a of the MST model can be taken as the adjusted number of theoretical plates of the affinity membranes. The maximum value of a was found experimentally by varying the flow rate; increasing the feed concentration led to a smaller value of alpha.
关键词[WOS]: AFFINITY-MEMBRANE ;  SORPTION KINETICS ;  CHROMATOGRAPHY ;  BIOSEPARATIONS ;  PROTEINS ;  BREAKTHROUGH ;  PURIFICATION ;  DESIGN
语种: 英语
WOS记录号: WOS:000224907300013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139496
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China

Recommended Citation:
Hao, WQ,Wang, JD,Li, JH. Modeling, by frontal analysis, of the adsorption of bovine serum albumin on cibacron blue-modified cellulose membranes[J]. CHROMATOGRAPHIA,2004,60(7-8):449-454.
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