DICP OpenIR
Modeling, by frontal analysis, of the adsorption of bovine serum albumin on cibacron blue-modified cellulose membranes
Hao, WQ; Wang, JD; Li, JH
KeywordMembrane-affinity Chromatography Frontal Analysis Cibacron Blue F3ga Bovine Serum Albumin
Source PublicationCHROMATOGRAPHIA
2004-10-01
DOI10.1365/s10337-004-0398-2
Volume60Issue:7-8Pages:449-454
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordAFFINITY-MEMBRANE ; SORPTION KINETICS ; CHROMATOGRAPHY ; BIOSEPARATIONS ; PROTEINS ; BREAKTHROUGH ; PURIFICATION ; DESIGN
AbstractThe adsorption of bovine serum albumin (BSA) on cibacron blue F3GA cellulose affinity membranes has been studied by frontal analysis. The ideal stirred tank (IST) model, the modified stirred tank (MST) model, and the equilibrium-dispersive (E-D) model were used to describe experimental breakthrough curves. The E-D model was found to best describe the initial region of the breakthrough curve whereas the MST model was best for the late region, implying a difference between the initial and late stages of adsorption. According to the E-D model, the effect of axial dispersion was not negligible, possibly because of experimental factors such as inhomogeneous distribution of the feed solution and the thinness of affinity membranes. The variable a of the MST model can be taken as the adjusted number of theoretical plates of the affinity membranes. The maximum value of a was found experimentally by varying the flow rate; increasing the feed concentration led to a smaller value of alpha.
Language英语
WOS IDWOS:000224907300013
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/139496
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China
Recommended Citation
GB/T 7714
Hao, WQ,Wang, JD,Li, JH. Modeling, by frontal analysis, of the adsorption of bovine serum albumin on cibacron blue-modified cellulose membranes[J]. CHROMATOGRAPHIA,2004,60(7-8):449-454.
APA Hao, WQ,Wang, JD,&Li, JH.(2004).Modeling, by frontal analysis, of the adsorption of bovine serum albumin on cibacron blue-modified cellulose membranes.CHROMATOGRAPHIA,60(7-8),449-454.
MLA Hao, WQ,et al."Modeling, by frontal analysis, of the adsorption of bovine serum albumin on cibacron blue-modified cellulose membranes".CHROMATOGRAPHIA 60.7-8(2004):449-454.
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