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题名: Analysis of coenzyme Q(10) in human plasma by column-switching liquid chromatography
作者: Ping, JA;  Wu, MH;  Zheng, YF;  Wang, C;  Li, YH;  Xin, JA;  Xu, GW
关键词: coenzyme Q(10)
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2004-06-15
DOI: 10.1016/j.jchromb.2004.03.008
卷: 805, 期:2, 页:297-301
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A new method of determining coenzyme Q(10) in human plasma was developed based on column-switching high performance liquid chromatography (HPLC). CoQ(10) was quantitatively extracted into I-propanol with a fast one-step extraction procedure, after centrifugation, the supernatant was cleaned on an octadecyl-bonded silica column and then transferred to reversed-phase column by a column-switching valve. Determination of CoQ(10) was performed on a reversed-phase analytical column with ultraviolet detection at 275 urn and the mobile phase containing 10% (v/v) isopropanol in methanol at a flow-rate of 1.5 ml/min. The sensitivity of this method allows the detection of 0.1 mug/ml CoQ(10) in plasma (S/N = 3). The linearity between the concentration and peak height is from 0.05 to 20 mg/l. The reproducibility (R.S.D.%) of the method is less than 2% (within day) and less than 3% (between day), the average recovery is 100.9 + 2.1%, it takes only 30 min to complete an analysis procedure, suitable for the determination of CoQ(10) in human plasma especially for batch analysis in clinical laboratories. Finally, the method was applied to determine the plasma CoQ(10) levels in healthy subjects, hyperthyroid and hypothyroid patients. (C) 2004 Elsevier B.V. All rights reserved.
关键词[WOS]: QUANTITATIVE-DETERMINATION ;  DIABETES-MELLITUS ;  Q(10) ;  UBIQUINONE ;  OXIDATION ;  REDUCTION ;  ASSAY
语种: 英语
WOS记录号: WOS:000221573700016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139531
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph R&A Ctr, Dalian 116011, Peoples R China
2.Dalian Ctr Hosp, Dalian 116033, Peoples R China
3.Dalian Univ Technol, Coll Chem, Dalian 116023, Peoples R China

Recommended Citation:
Ping, JA,Wu, MH,Zheng, YF,et al. Analysis of coenzyme Q(10) in human plasma by column-switching liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2004,805(2):297-301.
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