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题名: Development of functionalized fluorescent europium nanoparticles for biolabeling and time-resolved fluorometric applications
作者: Tan, MQ;  Wang, GL;  Hai, XD;  Ye, ZQ;  Yuan, JL
刊名: JOURNAL OF MATERIALS CHEMISTRY
发表日期: 2004
DOI: 10.1039/b407535h
卷: 14, 期:19, 页:2896-2901
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Technology
类目[WOS]: Chemistry, Physical ;  Materials Science, Multidisciplinary
研究领域[WOS]: Chemistry ;  Materials Science
英文摘要: A covalent binding-copolymerization method was developed to prepare silica-based fluorescent europium nanoparticles that can be used for biolabeling and highly sensitive time-resolved fluorescence bioassays. The nanoparticles were prepared in a water-in-oil (W/O) microemulsion consisting of a conjugate of (3-aminopropyl) triethoxysilane bound to a fluorescent Eu3+ chelate, 4,4'-bis(1", 1", 1", 2", 2", 3", 3"- heptafluoro-4", 6"-hexanedion-6"-yl) chlorosulfo-o-terphenyl- Eu3+ (APS-BHHCT-Eu3+), free (3-aminopropyl) triethoxysilane (APS), tetraethyl orthosilicate (TEOS), Triton X-100, n-octanol, water, and cyclohexane by copolymerization of APS-BHHCT-Eu3+, APS, and TEOS with aqueous ammonia. Characterization by transmission electron microscopy and fluorometric methods indicate that the nanoparticles are spherical and uniform in size, 36 +/- 4 nm in diameter, highly photostable, and strongly fluorescent, having a fluorescence quantum yield of 50.6% and a long fluorescence lifetime of 384 mus. The amino groups directly introduced to the surface of the nanoparticles by using free (3-aminopropyl) triethoxysilane in the nanoparticle preparation made the surface modification and bioconjugation of the nanoparticles easier. The nanoparticles were used for streptavidin labeling, and the nanoparticle-labeled streptavidin was used in sandwich-type time-resolved fluoroimmunoassays (TR-FIA) of carcinoembryonic antigens (CEA) and hepatitis B surface antigens (HBsAg) in human sera. The methods give detection limits of 1.9 pg ml(-1) for CEA, and 23 pg ml(-1) for HBsAg. The concentrations of HBsAg in 30 human serum samples were determined, and the results were compared with those independently determined by an established TR-FIA method using the BHHCT-Eu3+-labeled streptavidin. A good correlation was obtained with a correlation coefficient of 0.993.
关键词[WOS]: PROSTATE-SPECIFIC ANTIGEN ;  DOPED SILICA NANOPARTICLES ;  BIOANALYTICAL ASSAYS ;  LABEL TECHNOLOGY ;  CHELATE ;  IMMUNOASSAYS ;  PROTEIN ;  FLUOROIMMUNOASSAY ;  NANOCRYSTALS ;  COMPLEXES
语种: 英语
WOS记录号: WOS:000224174200012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/139538
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116012, Peoples R China

Recommended Citation:
Tan, MQ,Wang, GL,Hai, XD,et al. Development of functionalized fluorescent europium nanoparticles for biolabeling and time-resolved fluorometric applications[J]. JOURNAL OF MATERIALS CHEMISTRY,2004,14(19):2896-2901.
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